# Engineered AAV Identification, Validation, and Dissemination Pipeline for Brain Cell Type-Specific Manipulation Across Species

> **NIH NIH UF1** · CALIFORNIA INSTITUTE OF TECHNOLOGY · 2021 · $6,133,641

## Abstract

PROJECT SUMMARY:
This team initiative will provide the broad neuroscience community with a cell type-specific adeno-
associated virus (AAV) armamentarium for easy and non-invasive implementation of novel and
emerging molecular tools for anatomical and functional analysis of the nervous system in rodents,
non-human primates, and human organoids.
We will characterize engineered AAV capsids for cell type- and brain region-specific gene delivery
(Aim 1). We will pair these capsids with cargos of recently optimized and newly developed sensors
and effectors, largely enabled by the BRAIN Initiative, and validate their functional utility for large-
scale monitoring and manipulation of brain function across species (Aim 2). Finally, we will
establish an electronic AAV resource portal to catalogue and disseminate all of these tools to the
broader research community (Aim 3).
This proposal promises to validate a broad array of engineered systemic AAVs with access to
molecularly defined neuronal cell types for delivery of BRAIN-relevant cargo and functional
interrogation of brain cells across species. To maximize the utility of these tools to the
neuroscientific community, we will engage in broad collaboration and dissemination. Building on
the CLOVER Center’s established network of AAV production facilities, we will facilitate the broad
adoption of systemic AAVs through consultation and collaboration, including provision of cost-
free sample AAV preps. We will explore methodological innovations in AAV production and
administration while catalyzing the early-stage adoption and validation of engineered capsids
identified in this proposal by creating an electronic AAV resource for dissemination of reagents
and best practices, containing catalogued AAV brain atlases and a data re-capture portal for
continuous, crowd-sourced validation.
The expertise and infrastructure built over the life of this proposal will form the foundation of a
long-term comprehensive center for AAV BRAIN SENSUS (Safe and Effective Neuromodulator
and Sensor Utilization across Species) that will open new doors for neuroscience tool validation
and dissemination.

## Key facts

- **NIH application ID:** 10350260
- **Project number:** 1UF1MH128336-01
- **Recipient organization:** CALIFORNIA INSTITUTE OF TECHNOLOGY
- **Principal Investigator:** Andrew S Fox
- **Activity code:** UF1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $6,133,641
- **Award type:** 1
- **Project period:** 2021-09-20 → 2025-09-19

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10350260

## Citation

> US National Institutes of Health, RePORTER application 10350260, Engineered AAV Identification, Validation, and Dissemination Pipeline for Brain Cell Type-Specific Manipulation Across Species (1UF1MH128336-01). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10350260. Licensed CC0.

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