# Supplement: Regulation of Axonal Transport At Branch Junctions

> **NIH NIH R01** · THOMAS JEFFERSON UNIVERSITY · 2021 · $63,999

## Abstract

PROJECT ABSTRACT
 Axonal transport is essential to development and function of the nervous system. Axonal transport
relies on motor proteins (kinesins and dynein) to move protein, membrane and RNA cargos along microtubules.
It is especially important to long and often highly branched axons that requires building blocks made in the cell
body or signals received at axonal terminals to be transported for long distance. Recently studies have
identified many regulatory mechanisms, including the interactions between motor proteins with lattice-bound
microtubule associated proteins (MAPs), in different axonal regions. However, how axonal transport is
regulated to steer cargos into and out of branches is not well understood. This is an outstanding problem as
axonal branches are present throughout the nervous system. They not only define neuronal shape, but also
control synaptic connectivity and specificity, influence structural plasticity, and promote functional regeneration
after injury. The proposed study will tackle this under-studied problem by building on our long-term interest in
branch morphogenesis and cytoskeleton regulation as well as a recent discovery of a MAP in branch
development and transport regulation. Our preliminary data showed that transport at branch junctions is highly
selective as cargos are preferentially transported into growing branches. In addition, we also found that MAP7,
a MAP that is localized to branch junctions and interacts with the plus end motor kinesin-1, influences transport
behavior and branch growth. We thus hypothesize that axonal transport at branch junctions is controlled by a
selective routing mechanism that is mediated by specific motor-MAP interactions. To test this hypothesis, we
will: 1) establish a functional link between selective transport and branch growth; 2) dissect the mechanism
mediated by MAP7; and 3) establish selective routing as a common feature in axonal transport. By focusing
on an important region of the axon that has not been studied in the past, these studies will not only fill in a gap
in our understanding of axonal transport, but also provide new insights into synaptic development and function.
Given the importance of axonal transport in many neurological and neurodegenerative disorders, and the
association of MAP7 and kinesin-1 with epilepsy and ALS, our proposed studies of a basic neuronal cell
biological problem will provide new knowledge to uncover disease mechanisms, and thus are highly relevant to
the NIH mission to understand and enhance human health.

## Key facts

- **NIH application ID:** 10354520
- **Project number:** 3R01NS114247-02S1
- **Recipient organization:** THOMAS JEFFERSON UNIVERSITY
- **Principal Investigator:** Le Ma
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $63,999
- **Award type:** 3
- **Project period:** 2020-04-01 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10354520

## Citation

> US National Institutes of Health, RePORTER application 10354520, Supplement: Regulation of Axonal Transport At Branch Junctions (3R01NS114247-02S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10354520. Licensed CC0.

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