# Organoid Cultivation Core

> **NIH NIH U19** · BAYLOR COLLEGE OF MEDICINE · 2022 · $400,444

## Abstract

PROJECT SUMMARY
The goal of this U19 Combating Antibiotic Resistant Bacteria (CARB) Interdisciplinary Research Unit (CARBIRU)
application is to gain a deeper understanding of bacteria and host factors that play a role in antibacterial
resistance and infection. To meet this goal, the establishment of the Organoid Cultivation Core (OCC) will allow
the provision of critical biologically relevant human organotypic cultures that are essential for the success of the
project. The OCC will be organized around two goals: 1) development of human derived organoids from the
vagina, the bladder and the nasal airway for use by the individual projects and (2) production of organoids for
each project to assist in meeting their individual stated research goals. These goals will offer the ability to test
key hypotheses about the role of the host epithelium and develop key outcomes that can be translated into
understanding bacterial infections and antibacterial resistance. The core will reduce labor, supply and equipment
costs, and time required to complete projects by centralizing the isolation, growth, and maintenance of the
organoid lines. Bulk acquisition of materials required to culture organoids, standardization of conditioned media
containing growth factors, and centralization of the maintenance of the lines result in significant time and cost
savings to the individual Project investigators and ensures standardization and reproducibility. Additionally, the
OCC core will enhance communication and comparability of results obtained between projects. The OCC core
will be an essential component of the success of each of the projects in this application as well as a unifying
element in connecting them together.

## Key facts

- **NIH application ID:** 10357967
- **Project number:** 5U19AI157981-02
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** SARAH E BLUTT
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $400,444
- **Award type:** 5
- **Project period:** 2021-03-01 → 2026-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10357967

## Citation

> US National Institutes of Health, RePORTER application 10357967, Organoid Cultivation Core (5U19AI157981-02). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10357967. Licensed CC0.

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