# Reducing Inflammation After Spinal Cord Injury Via Mineral Coated Microparticles Releasing Anti-Inflammatory Cytokines

> **NIH NIH R56** · UNIVERSITY OF WISCONSIN-MADISON · 2021 · $387,500

## Abstract

Project Summary / Abstract:
Significance: Spinal Cord Injury (SCI) is a devastating trauma that leaves approximately 10,000 to 20,000
people paralyzed every year in the U.S., costing the health care system $40.5 billion annually. Although it has
been shown that the inflammatory response after SCI is beneficial in removing debris and releasing neurotrophic
factors, there is an overreaction of the inflammatory response causing further neural destruction and
inflammatory macrophages remain for a prolonged time period. Inflammatory cytokines are strongly upregulated
during the first 24 hours after SCI, and there is a second wave of inflammatory cytokine expression around 14
days. Although, using anti-inflammatory cytokines to attenuate inflammation after SCI has shown some
encouraging results, there are several limitations that need to be overcome to use anti-inflammatory cytokines
as a treatment for SCI including, a short half-life, inability to cross the blood spinal cord barrier, rapid clearance
from the injury site, and higher risk of infection when using large systemic doses. Therefore, it would be beneficial
to have a local sustained delivery of anti-inflammatory cytokines, coinciding with critical stages of the ensuing
inflammatory response, given directly in the injury site for at least 14 days.
Innovation: Emerging cytokine delivery approaches are often limited by sub-optimal release characteristics and
poor biological activity of the cytokine when delivered in vivo. We hypothesize that mineral coated microparticles
(MCMs) will bind, stabilize and release biologically active anti-inflammatory cytokines, and when injected at
clinically relevant times with optimized dose and release profile, they will reduce inflammation resulting in smaller
lesions and a higher level of function retained after SCI.
Aims: The proposed plan is to 1) develop a cytokine delivery system using MCMs, and characterize
incorporation, release, and biological activity of IL-4, IL-10, and IL-13; 2) optimize cytokine dosage and treatment
timeline in a rat contusion model; and 3) explore the synergistic effects of combinations of cytokines to reduce
inflammation and improve the amount of function retained below the level of injury after SCI.
Impact: Successful completion of the proposed research program will produce three direct outcomes. First, it
will establish a method to attenuate inflammation after SCI using locally delivered biologically active anti-
inflammatory cytokines with optimized dosage and timing. Second, it will explore fundamental synergies between
cytokines that influence specific stages of inflammation. Third, it will develop a controllable drug delivery system
for biologically active molecules after SCI. In view of the importance of soluble cytokines involved in the
inflammatory response after SCI, as well as growth factors that promote signaling and guidance cues for axonal
growth, the proposed MCMs are expected to be a uniquely enabling technology in ...

## Key facts

- **NIH application ID:** 10357988
- **Project number:** 1R56NS117935-01A1
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** Amgad S Hanna
- **Activity code:** R56 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $387,500
- **Award type:** 1
- **Project period:** 2021-05-01 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10357988

## Citation

> US National Institutes of Health, RePORTER application 10357988, Reducing Inflammation After Spinal Cord Injury Via Mineral Coated Microparticles Releasing Anti-Inflammatory Cytokines (1R56NS117935-01A1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10357988. Licensed CC0.

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