# Bioengineering approaches to map mechanotransduction in the living cell

> **NIH NIH R01** · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · 2022 · $389,025

## Abstract

Project Summary/Abstract
It is increasingly evident that both soluble factor-mediated chemical signaling and physical force-mediated
mechanotransduction play critical roles in living cells and tissues. Yet we know relatively little about how force
regulates gene expression and vital biological functions. Mechanosensors at or near the cell surface such as
integrin, talin, or vinculin have been reported, but whether the nucleus itself and its chromatin can act as a
mechanosensor is an unanswered question in the field of mechanobiology. Yet regulation of gene expression in
normal cells and cancer cells is one of the most critical processes that control cellular functions and behaviors.
This research project aims to understand how forces and mechanical microenvironment directly influence gene
expression and to identify intra-nuclear proteins that are responsible for mediating force transfer from nuclear
lamina to lamina-associated domains of chromatin in living cells. Our preliminary results strongly suggest that a
local physiologically-relevant force via integrins can directly upregulation transcription via stretching the
chromatin in living cells. Built on these results, we propose 3 specific aims to elucidate mechanotransduction
mechanisms in the living cells. Aim 1: to test the hypothesis that chromatin stretching is required for direct
transcription upregulation by force via integrins; Aim 2: to test the hypothesis that force via integrins directly
activates mechanosensitive genes more than housekeeping gene DHFR; Aim 3: to dissect out the mechanism
of gene repression in response to high forces and on stiff matrices. Our experimental designs are rigorous and
the likelihood of generating insightful discovery is very high. The long-term goal is to develop novel strategies to
intervene the processes that regulate gene expression in living cells in animals and human subjects to treat and
cure diseases like malignant tumors.

## Key facts

- **NIH application ID:** 10359167
- **Project number:** 5R01GM072744-18
- **Recipient organization:** UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
- **Principal Investigator:** Ning Wang
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $389,025
- **Award type:** 5
- **Project period:** 2005-08-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10359167

## Citation

> US National Institutes of Health, RePORTER application 10359167, Bioengineering approaches to map mechanotransduction in the living cell (5R01GM072744-18). Retrieved via AI Analytics 2026-05-21 from https://api.ai-analytics.org/grant/nih/10359167. Licensed CC0.

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