# Automated CRISPR Enabled DNA Synthesis (CEDS)

> **NIH NIH R21** · DUKE UNIVERSITY · 2022 · $295,025

## Abstract

Abstract
We propose to develop a novel method for low cost DNA synthesis: CEDS (Crispr enabled DNA
synthesis). The CEDS approach overcomes many of these challenges and enables template
independent, exponential single- stranded DNA synthesis. We have preliminary results
supporting key biochemical steps in the CEDS workflow, including both ssDNA ligation and Cpf1
mediated ssDNA cleavage at the target strand. Our longer term goal is to develop the technology
to support the overnight synthesis of a 10 kbp plasmid at a cost of <$10.00 which would be
$0.001/bp in synthesis cost (not including the costs of sequence verification).

## Key facts

- **NIH application ID:** 10369127
- **Project number:** 1R21HG011675-01A1
- **Recipient organization:** DUKE UNIVERSITY
- **Principal Investigator:** Michael David Lynch
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $295,025
- **Award type:** 1
- **Project period:** 2022-02-08 → 2024-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10369127

## Citation

> US National Institutes of Health, RePORTER application 10369127, Automated CRISPR Enabled DNA Synthesis (CEDS) (1R21HG011675-01A1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10369127. Licensed CC0.

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