# Structure and Function of Metabolic Filaments

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2022 · $367,751

## Abstract

PROJECT SUMMARY
Intermediate metabolism must be finely tuned, carefully balanced, and robustly adaptable to changes in
environmental conditions. While the individual enzymes that drive most metabolic processes are well
understood, only recently has the field come to appreciate the widespread role of metabolic enzyme self-
assembly in metabolic organization and control. In particular, we now know that dozens of metabolic enzymes
assemble into filamentous structures in vivo and in vitro, and that these filaments act as allosteric effectors to
tune enzyme activity. This proposal focuses on self-assembled filaments of metabolic enzymes in glycolysis and
nucleotide biosynthesis, processes that extract energy from sugar and generate fundamentally important
macromolecular building blocks, respectively. We will use cryo-electron microscopy to determine the structural
basis for assembly and regulation, coupled with biochemical, biophysical, and cell biological approaches in an
integrative approach to understanding metabolic filament function. This work will provide insight into the specific
roles polymerization plays in modulating enzyme function, and illuminate general principles of metabolic control
by enzyme filaments.

## Key facts

- **NIH application ID:** 10370322
- **Project number:** 5R01GM118396-07
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Justin M Kollman
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $367,751
- **Award type:** 5
- **Project period:** 2016-04-01 → 2025-02-28

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10370322

## Citation

> US National Institutes of Health, RePORTER application 10370322, Structure and Function of Metabolic Filaments (5R01GM118396-07). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10370322. Licensed CC0.

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