# Method development for simultaneous automatic assignment and structure determination in protein NMR

> **NIH NIH R21** · RENSSELAER POLYTECHNIC INSTITUTE · 2022 · $223,395

## Abstract

Protein NMR is a powerful tool for studying protein structure, dynamics and interactions at
atomic resolution. However, currently it is time-consuming and laborious to obtain NMR
resonance assignment and the NMR structure of a protein. Here we will leverage recent
advances in Euclidean distance geometry to develop a method for simultaneous
determination of resonance assignment and protein structure using only the NOESY spectra.
We have two aims: 1. Method development and validation using synthetic data based on
known protein structure; 2 Method development and validation using experimental NMR
data.

## Key facts

- **NIH application ID:** 10373305
- **Project number:** 1R21GM143507-01A1
- **Recipient organization:** RENSSELAER POLYTECHNIC INSTITUTE
- **Principal Investigator:** Rongjie Lai
- **Activity code:** R21 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $223,395
- **Award type:** 1
- **Project period:** 2022-09-12 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10373305

## Citation

> US National Institutes of Health, RePORTER application 10373305, Method development for simultaneous automatic assignment and structure determination in protein NMR (1R21GM143507-01A1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10373305. Licensed CC0.

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