# Development of a high performance, compact Snapshot Hyperspectral Camera with light integrator array

> **NIH NIH R43** · ATTORIS, LLC · 2022 · $252,041

## Abstract

Development of a high performance, compact Snapshot Hyperspectral Camera with light integrator array.
The overall goal of this research is to develop a compact, high dynamic range, snapshot hyperspectral camera
for biomedical and biological applications. The technology is based on utilization of a TApered Light Integrator
Array for Imaging Spectrometry. For the purposes of this application and name simplification we call the system
TALIARIS. As the proposed development is a platform technology (complete system can be treated as a spectral
camera easily coupled to microscopes as well as to endoscopes, fundus cameras and other imaging
instruments) it can be used in fundamental cell signaling studies as well as medical diagnostics for example
retinal imaging / cancer diagnostics. In this proposal though, the experimental focus will be limited to cell signaling
in microscopic imaging.
The proposed system offers faster frame rates and higher dynamic range (by 1+ orders of magnitude) than other
current methods for hyperspectral and multi-spectral imaging of living systems, while still permitting diffraction-
limited resolution. Snapshot feature in combination with high light collection efficiency (based on unique, recently
patented array of light integrators for spectral imaging) greatly enhances a range of possible biological
experiments (its characteristics include also low photobleaching, increased patient comfort at lower light
intensities, etc.). The TALIARIS instrument is fully parallel so data at all wavelengths will be collected
simultaneously from the entire field of view. Thus this approach has a great potential to overcome limitations of
existing hyperspectral modalities, and enables real time imaging of multiple signaling processes in living
systems.
Towards this goal, our first aim will focus on the development of a proof of concept TALIARIS system and a
prototype of array of miniature light integrators. The TALIARIS will transform a 3D spatial-spectral object cube
to a 2D mapped image, which allows acquisition of entire 3D data cube in the snapshot mode. The core of the
system is the custom-made array of miniature light integrators allowing collection of all light at the input and
concentrating it at smaller output areas to create void spaces needed for spectral information. For low noise
imaging, the proposed device will employ IRIS 15 camera from Photometrics (15Mpix sCMOS camera). The
TALIARIS system will be optimized for throughput and will permit imaging in real time at 30+ frames/second,
and 16-bit dynamic range. The proposed system, we will provide datacube dimensions spanning from
100x100x25 to 250x250x50 with spectral sampling of 5 nm over 470 – 670 spectral range. Spatial resolution and
FOV are inherently connected with fore-optics and will depend on specific microscope objective being used in
experiments. TALIARIS will be validated in microscopic imaging to obtain effective spatial resolution of 0.5
micron and 125 microns FOV.
...

## Key facts

- **NIH application ID:** 10384195
- **Project number:** 1R43GM142353-01A1
- **Recipient organization:** ATTORIS, LLC
- **Principal Investigator:** ARTUR G OLSZAK
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $252,041
- **Award type:** 1
- **Project period:** 2022-05-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10384195

## Citation

> US National Institutes of Health, RePORTER application 10384195, Development of a high performance, compact Snapshot Hyperspectral Camera with light integrator array (1R43GM142353-01A1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10384195. Licensed CC0.

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