# Equipment Supplement: Analysis tools for quantifying protein conformational landscapes using DEER spectroscopy

> **NIH NIH R01** · UNIVERSITY OF WASHINGTON · 2021 · $91,677

## Abstract

PROJECT SUMMARY/ABSTRACT
This project develops new computational analysis and modeling methods for DEER (double electron-electron
resonance) spectroscopy. DEER is a biostructural technique for the quantification of protein conformational
landscapes and protein motions on the nanometer scale. Protein motions are crucial for many key molecular
processes at the basis of human life and disease. Therefore, DEER provides important insights that contribute
to the knowledge base necessary for drug development. In combination with X-ray crystallography, NMR and
cryo-EM, DEER is part of a complementary set of integrative experimental biostructural tools. It is especially
important for the study of membrane proteins. A major barrier in the field is the lack of integrated analysis tools
for biomedical researchers. This project addresses this issue by (a) developing tools based on Bayesian statistics
for the rigorous and reproducible analysis of experimental DEER data, providing comprehensive methods for
uncertainty quantification (error bars) in DEER data, and (b) creating advanced computational tools to utilize
DEER data in the refinement of protein structures based on atomic-resolution ensemble models. Overall, the
goal of the project is to significantly accelerate the workflow of DEER data analysis, interpretation and modeling
and increase its rigor, reproducibility and accessibility. This will enable the study of the structure and dynamics
of larger and more complex proteins and protein assemblies, which are increasingly important in biomedical
research.

## Key facts

- **NIH application ID:** 10385498
- **Project number:** 3R01GM125753-03S1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Stefan Stoll
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $91,677
- **Award type:** 3
- **Project period:** 2019-02-01 → 2023-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10385498

## Citation

> US National Institutes of Health, RePORTER application 10385498, Equipment Supplement: Analysis tools for quantifying protein conformational landscapes using DEER spectroscopy (3R01GM125753-03S1). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10385498. Licensed CC0.

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