# A Novel Targeted Therapy for Subarachnoid Hemorrhage

> **NIH NIH R43** · LOXAGEN INC. · 2022 · $256,386

## Abstract

Summary
The goal of this project is to develop a therapeutic capable of treating subarachnoid hemorrhage
(SAH), a severe form of stroke characterized by bleeding into the subarachnoid space. Around
30,000 cases of spontaneous SAH occur in the US population every year. They are typically
caused by a ruptured cerebral aneurysm, whose risk factors include, high blood pressure,
smoking, family history and alcoholism. Aneurysmal SAH is associated with high mortality: 50%
of patients die within 30 days, and of the survivors many retain neurological deficits. We have
recently shown that the lipoxygenase ALOX15 is increased in macrophages adjacent to the
subarachnoid blood in a mouse model of SAH, contributing to early brain injury. SAH induced
brain edema was ALOX15 dependent and ALOX15 gene knockout reduced neuronal cell death
and improved behavioral outcome. Loxagen’s lead compound, BPN-27332, selectively inhibits
ALOX15 with high potency (IC50 = 80 nM) and can reduce neuronal cell death and improve
neurological outcome after SAH. In this project, we will pursue the following aims: Aim 1:
Synthesize sufficient amounts of BPN-27332 to support in vivo studies. Aim 2: Determine the
most effective dose, confirm target engagement by lowering ALOX15 product and determine the
optimal time window for treatment. These results will allow us to apply for a Phase II SBIR
where we will expand our investigations and develop BPN-27332 as novel therapeutic to treat
the brain injury resulting from subarachnoid hemorrhage.

## Key facts

- **NIH application ID:** 10385590
- **Project number:** 1R43NS124491-01A1
- **Recipient organization:** LOXAGEN INC.
- **Principal Investigator:** Kalidip Choudhury
- **Activity code:** R43 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $256,386
- **Award type:** 1
- **Project period:** 2022-09-01 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10385590

## Citation

> US National Institutes of Health, RePORTER application 10385590, A Novel Targeted Therapy for Subarachnoid Hemorrhage (1R43NS124491-01A1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10385590. Licensed CC0.

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