# Defining the conformational control of nitric oxide synthases by a multipronged approach

> **NIH NIH R01** · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · 2021 · $105,024

## Abstract

PROJECT SUMMARY
The parent award is on deciphering conformational dynamics of nitric oxide synthase proteins through an
integrated approach using advanced spectroscopic techniques. Detailed spectroscopic studies require
milligram amounts of highly purified protein. Fast protein liquid chromatography (FPLC) is a form of liquid
chromatography used to purify a target protein from a complex mixture. Our current FPLC has been a
workhorse, but is near the end of operational lifetime. This administrative supplement is being requested to
allow acquisition of a state-of-the-art ÄKTA pure 25 system. It has direct relevance to and immediate utility in
enhancing our laboratory’s protein purification capabilities. As such, this equipment will greatly enhance our
research productivity for the duration of the project, and will help us maintain a strong research program.
Importantly, the unique capabilities of the ÄKTA pure 25 system will be indispensable for our efforts to develop
and optimize methods for site-specifically incorporating unnatural amino acids into large, complex proteins with
high fidelity, yield, and purity. Our findings will therefore be of great utility for other investigators using this
process for other large, complex enzymes. Therefore, the requested equipment will help the parent project to
exert a sustained, powerful influence on the research fields of bioinorganic and biophysical chemistry.
Moreover, the administrative supplement will enhance the scientific environment for NIH-funded investigators
at the University of New Mexico, both within and outside of the College of Pharmacy. To our knowledge, no
comparable instrumentation exists in educational institutions within the region. Dean Godwin has committed
substantial financial support to this supplement request since this is a great investment for the College. Taken
as a whole, the proposed supplement will increase the parent award’s overall impact within the original scope
of award, as well as bolster research quality and depth at our institution.

## Key facts

- **NIH application ID:** 10385652
- **Project number:** 3R01GM133973-02S1
- **Recipient organization:** UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR
- **Principal Investigator:** Changjian (Jim) Feng
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $105,024
- **Award type:** 3
- **Project period:** 2020-08-01 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10385652

## Citation

> US National Institutes of Health, RePORTER application 10385652, Defining the conformational control of nitric oxide synthases by a multipronged approach (3R01GM133973-02S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10385652. Licensed CC0.

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