# Protein purification instrumentation in support of single molecule studies of genome integrity

> **NIH NIH R35** · COLUMBIA UNIVERSITY HEALTH SCIENCES · 2021 · $93,402

## Abstract

My group has a long–standing interest in understanding the molecular mechanisms that
contribute to cancer biology and genome integrity. To provide new insights into the mechanisms
of genome integrity we have pioneered the development of DNA curtains, which allow for the
direct visualization of hundreds to thousands of individual molecules in real–time by optical
microscopy. This experimental platform is applicable to many types of protein–nucleic acid
interactions and allows us to address questions about complex biological problems that cannot
be addressed through traditional approaches. Our success in with this technology has uniquely
positioned the lab to reveal new concepts regarding the mechanisms of genome integrity. Our
use of this novel single molecule methodology is dependent upon access to highly purified
recombinant proteins. This Supplemental Equipment request will allow us to replace our
outdated protein purification system so that we can continue moving forward on our research
efforts into understanding the mechanisms of genome integrity.

## Key facts

- **NIH application ID:** 10386035
- **Project number:** 3R35GM118026-06S1
- **Recipient organization:** COLUMBIA UNIVERSITY HEALTH SCIENCES
- **Principal Investigator:** Eric C Greene
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $93,402
- **Award type:** 3
- **Project period:** 2021-05-01 → 2022-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10386035

## Citation

> US National Institutes of Health, RePORTER application 10386035, Protein purification instrumentation in support of single molecule studies of genome integrity (3R35GM118026-06S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10386035. Licensed CC0.

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