# Regulation of Chemotactic Signaling

> **NIH NIH R35** · JOHNS HOPKINS UNIVERSITY · 2021 · $126,159

## Abstract

Summary
Through the funding opportunity described in PAR-20-272, this proposal seeks funds to
purchase a fluorescence-detection HPLC (high-performance liquid chromatography) system to
quantitatively assess stimulation-induced dynamic assembly of protein complexes in
chemotactic signaling. The goals of the parent grant R35GM131768 are to investigate how cells
sense extracellular chemical gradients and control their migration behaviors. First, we will
determine how phosphorylated, GDP-bound RacE activates TORC2 to facilitate AKT
phosphorylation at the leading end of migrating cells while GTP-bound RacE inhibits TORC2 at
the trailing end. This will involve multiple experimental approaches, including cellular
reconstitution using knockout cell lines, biochemical reconstitution of RacE-regulated TORC2
activity with purified proteins, protein interaction analyses at the single-molecule level in vitro,
live imaging of cells, and structural analysis using X-ray crystallography. Second, we will
decipher a key mechanism controlling human PTEN localization at the plasma membrane and
nucleus. Third, we will determine the effects of the manipulation of PTEN localization on
tumorigenesis in vivo using animal models.

## Key facts

- **NIH application ID:** 10386438
- **Project number:** 3R35GM131768-03S1
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** Miho Iijima
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $126,159
- **Award type:** 3
- **Project period:** 2019-06-01 → 2024-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10386438

## Citation

> US National Institutes of Health, RePORTER application 10386438, Regulation of Chemotactic Signaling (3R35GM131768-03S1). Retrieved via AI Analytics 2026-06-04 from https://api.ai-analytics.org/grant/nih/10386438. Licensed CC0.

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