# The Jackson Laboratory Knockout Mouse Production and Phenotyping Project (JAX KOMP2)

> **NIH NIH UM1** · JACKSON LABORATORY · 2021 · $2,391,665

## Abstract

PROJECT SUMMARY
The goal of The Jackson Laboratory (JAX) Knockout Mouse Phenotyping Project phase 2 (KOMP2) is to
contribute to the international effort of creating a comprehensive catalog of mammalian gene function obtained
through the creation of gene knockouts and execution of a high-throughput phenotyping pipeline. At JAX an
Early Adult Pipeline (EAP) is being used to phenotype mice up to 17 weeks of age with a goal of screening 1,000
mutant lines by the end of the current 5-year project period. Further, during this award period, a subset of 150
lines is being aged out to 18 months and phenotyped using a Late Adult Pipeline (LAP) to detect age-associated
phenotypes. Nonviable and subviable lines are also being phenotyped using specialty embryonic phenotyping
pipelines. On March 11, 2020 the World Health Organization (WHO) declared the outbreak of severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) a world-wide pandemic. A consequence
of the pandemic has been a reduction of our available personnel and services needed to reach our original goals.
Restrictions during the pandemic have curtailed the breeding and phenotyping activity on the JAX KOMP2 center.
We have maintained a lower level of breeding of cohorts and reduced phenotyping of mice. In addition, in order
to maximize physical distancing and operate in a way that mitigates viral transmission and spread, we have
discontinued procedures that require close contact between staff. Due to the pandemic, we are short of the total
number of lines projected to have been phenotyped and on the number of lines with full cohorts for all tests. The
purpose of this request for a funded extension beyond the current project period is to complete the initial goals
of creating and phenotyping 1000 lines of KO mice. We project that at the end of the original grant period that
860 lines of mice will have completed the EAP pipeline and that LAP phenotyping data from all 150 lines will be
collected. The Specific Aims of this funded extension are to breed and populate the EAP from the remaining 140
mouse lines to bring our total to 1000 lines. Approximately 70% of the lines (~100 lines) will be fully viable and
will therefore be phenotyped as homozygotes. The remaining 30% of the lines (~40 lines) be non-viable or
subviable and will therefore enter the EAP as heterozygotes. These 40 lines will also be used to populate our
embryo lethal phenotyping pipeline. QC analysis will be performed on both the EAP and LAP data and exported
to the Data Coordination Center of the International Mouse Phenotyping Consortium.

## Key facts

- **NIH application ID:** 10386984
- **Project number:** 3UM1OD023222-10S2
- **Recipient organization:** JACKSON LABORATORY
- **Principal Investigator:** ROBERT E BRAUN
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $2,391,665
- **Award type:** 3
- **Project period:** 2021-04-11 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10386984

## Citation

> US National Institutes of Health, RePORTER application 10386984, The Jackson Laboratory Knockout Mouse Production and Phenotyping Project (JAX KOMP2) (3UM1OD023222-10S2). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10386984. Licensed CC0.

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