# Understanding Cell Division

> **NIH NIH R35** · OKLAHOMA MEDICAL RESEARCH FOUNDATION · 2021 · $11,220

## Abstract

Summary
The parent grant (1R35GM126980) for this equipment request supplement is entitled
“Understanding Cell Division.” This award covers the primary research focus of the
Gorbsky laboratory, analyzing the mechanics of chromosome movement, the regulation
of the mitotic cell cycle, and the characterization of factors and conditions that contribute
to chromosome instability. Much of our research involves high resolution imaging of
cultured cells expressing fluorescently tagged proteins and are manipulated with RNA
interference or CRISPR gene knockout and mutation. The Mizar Tilt light sheet provides
high resolution imaging with minimal phototoxicity. The approaches used in the lab to
interrogate protein function include siRNA or knocking degron/GFP tags into endogenous
genes. We are also using the “Trim-away” technique, through which a combination of
antibody and Trim13 E3 ubiquitin ligase, microinjected into living cells, allows rapid
degradation of endogenous proteins without genetic manipulations. The requested
upgrade to our Mizar Tilt high resolution light sheet, the recently introduced Tilt-RM
resonance mirror, microscope will reduce “striping” an inherent defect in light sheet
microscopy. This modification will greatly enhance the imaging capabilities of the system,
particularly for quantitative fluorescence measurements.

## Key facts

- **NIH application ID:** 10387165
- **Project number:** 3R35GM126980-04S1
- **Recipient organization:** OKLAHOMA MEDICAL RESEARCH FOUNDATION
- **Principal Investigator:** GARY J. GORBSKY
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $11,220
- **Award type:** 3
- **Project period:** 2018-07-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10387165

## Citation

> US National Institutes of Health, RePORTER application 10387165, Understanding Cell Division (3R35GM126980-04S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10387165. Licensed CC0.

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