# Tissue repair, extracellular vesicular biogenesis, and the control of immune responses

> **NIH NIH R01** · UNIVERSITY OF CALIFORNIA, SAN DIEGO · 2021 · $219,620

## Abstract

SUMMARY
Resolution in skin wound healing is coordinated by intercellular communication processes that
include extracellular vesicles (EVs) containing biologically active protein and nucleic acid. While
EV biogenesis has been studied in vitro, systematic approaches to identify their in vivo relevance
are limited. Having established that the expression of TBC/RabGAPs controls EV biogenesis and
‘re-programs’ EV content to uncouple the pro-reparative activities of EVs in inflammation, we
propose to use a genetic approach to target the secretory machinery implicated in EV biogenesis
(i.e. Rabs 5, 11, and 27A) to determine how these targets of TBC/RabGAPs regulate the payload
of EVs. We will determine how EVs, and changes in EVs, impact the resolution of inflammation,
and gain insight into the role of EVs in wound healing. In Aim 1, we will use vesicle flow
cytometry to define EV subsets, followed by adoptive transfer of EVs to determine their capacity
to activate specific immune cell populations in diabetic and immunodeficient knockout mouse
models that mediate cutaneous wound closure. Aim 2 will study specific Rabs to determine how
these Rabs impact EV biogenesis and content. In addition, using the EVs formed in the altered
Rab environment, we will establish the mechanism of action and effect of these EVs on the
kinetics of resolution of specific immune responses. Together, these studies will establish the
relevance of EVs as potential therapeutics and address the over-arching goal of systematically
creating therapeutic EVs that are specifically optimized for specific phases of skin repair.

## Key facts

- **NIH application ID:** 10387452
- **Project number:** 3R01GM140137-02S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA, SAN DIEGO
- **Principal Investigator:** Brian P Eliceiri
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $219,620
- **Award type:** 3
- **Project period:** 2020-09-20 → 2024-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10387452

## Citation

> US National Institutes of Health, RePORTER application 10387452, Tissue repair, extracellular vesicular biogenesis, and the control of immune responses (3R01GM140137-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10387452. Licensed CC0.

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