# Substrate targeting mechanism of a DesCEND pathway

> **NIH NIH R01** · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · 2021 · $70,340

## Abstract

Abstract
This request for a supplement to the grant R01GM135592 proposes the acquisition of a liquid chromatography
protein purification system to support the research aims that focus on investigating the substrate targeting
mechanisms of the F-box subunit FBXO31 of the SCF ubiquitin ligase in a novel DesCEND pathway. By
identifying and characterizing substrates of FBXO31 at both a systems level and the molecular level, we
proposed to elucidate the complementary structural determinants of the FBXO31–degron pair that confer
substrate specificity and dictate protein recognition and fate. To achieve our goals, it is vital that we have the
ability to obtain highly purified protein samples from complex starting materials derived from cell lysates and
recombinant protein expression systems. This proposal requests funds to replace a 14-year old (and recently
defunct) AKTA FPLC protein purification instrument with a similar but up-to-date version from the same
manufacturer. The proposed system, the AKTA pure, is a versatile and powerful liquid chromatography system
for the reliable purification of proteins and protein complexes. It covers a broad range of chromatography
applications and meets the automation requirements needed to deliver the highest purity. The instrument will
ensure the continued biochemical, biophysical and structural studies proposed in the parent award.

## Key facts

- **NIH application ID:** 10387550
- **Project number:** 3R01GM135592-02S1
- **Recipient organization:** UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
- **Principal Investigator:** Bing Hao
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $70,340
- **Award type:** 3
- **Project period:** 2020-08-15 → 2024-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10387550

## Citation

> US National Institutes of Health, RePORTER application 10387550, Substrate targeting mechanism of a DesCEND pathway (3R01GM135592-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10387550. Licensed CC0.

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