# Molecular Mechanisms of Replication Stress Response

> **NIH NIH R35** · UNIVERSITY OF SOUTH FLORIDA · 2021 · $46,410

## Abstract

PROJECT SUMMARY
This is a shared instrumentation NOSI grant application at the University of South Florida (USF)
to request FiberVision S automatic scanner developed by Genomic Vision. The PIs are part of
the Genome Integrity and Mechanisms of Aging focus group within the Cell Biology, Microbiology
and Molecular Biology department. The primary research focus of my research group is to
delineate genome maintenance mechanisms that promote DNA duplication and genome stability
when cells are challenged with replication stress. The collective research areas of both the
research teams will benefit from the instrument that will allow high throughput studies on genome
duplication and integrity in human cells. The versatile fluorescence scanner can be utilized for a
number of applications including DNA replication dynamics (origin firing, replication elongation,
stalled fork stability studies, fork restart studies) and chromosomal rearrangement analyses
(deletions, copy number variations, inversions, translocations). An added advantage is to combine
DNA combing experiments with FISH hybridization to interrogate genomic-loci specific changes
in DNA replication. Although the research programs are diverse, the overarching goal of the
investigators is to explore the relevance of genome duplication events to human diseases such
as Blooms syndrome and cancer. An automated fluorescence microscope with the self-aided
capabilities of scanning, image acquisition and data analyses will substantially benefit our
research program and further our ability to maximize research output.

## Key facts

- **NIH application ID:** 10387590
- **Project number:** 3R35GM137800-02S1
- **Recipient organization:** UNIVERSITY OF SOUTH FLORIDA
- **Principal Investigator:** Huzefa Mannan Dungrawala
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $46,410
- **Award type:** 3
- **Project period:** 2020-07-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10387590

## Citation

> US National Institutes of Health, RePORTER application 10387590, Molecular Mechanisms of Replication Stress Response (3R35GM137800-02S1). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10387590. Licensed CC0.

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