# Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition

> **NIH NIH R01** · UNIVERSITY OF MISSOURI-COLUMBIA · 2021 · $92,072

## Abstract

This proposal requests funds for a high-performance liquid chromatograph (HPLC) that is suitable for both
analytical and preparative work, as a major instrumentation supplement in support of the parent grant
R01GM125669, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition. A highcaliber HPLC is a critical piece of standard laboratory equipment for almost any cutting edge biochemical
research. This instrument will be used in preparative capacity for purification of chemically-modified
oligonucleotides (such as dye-labeled primers and site-specifically modified DGR cDNA
intermediates), proteins (such as bRT and bAvd from Bordatella phage and from phylogenetically related
phage), and specialty small molecules (such as modified nucleotide analogs). We would also use the
instrument in analytical capacity for quantitative assessment of chemical purity of reaction products
involving small molecules, macromolecules, and conjugated variants of the macromolecules.

## Key facts

- **NIH application ID:** 10387714
- **Project number:** 3R01GM125669-04S1
- **Recipient organization:** UNIVERSITY OF MISSOURI-COLUMBIA
- **Principal Investigator:** Donald H Burke
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $92,072
- **Award type:** 3
- **Project period:** 2018-09-19 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10387714

## Citation

> US National Institutes of Health, RePORTER application 10387714, Mechanism of Microbial DNA Hypervariation through Mutagenic Transposition (3R01GM125669-04S1). Retrieved via AI Analytics 2026-06-01 from https://api.ai-analytics.org/grant/nih/10387714. Licensed CC0.

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