# DNA helicases and associated factors in genome stability

> **NIH NIH R35** · TRUSTEES OF INDIANA UNIVERSITY · 2021 · $59,139

## Abstract

PROJECT SUMMARY
 DNA helicases function in virtually all aspects of DNA replication, recombination, and repair. As such,
they are vital to maintaining genome integrity and are disease linked when mutated. Despite many in vivo and
in vitro advances in working with helicases, there is a gap in knowledge connecting mutant alleles of helicase
genes to the treatment of patients in clinics. The objective of my research is to gain mechanistic insight into
how DNA helicases function in genome maintenance and why their dysfunction leads to disease. Toward this
goal, we are studying PIF1 and RecQ family helicases, which are evolutionarily conserved and because
mutations in the human genes encoding these helicases are associated with multiple diseases. Our current
work focuses on the roles of RecQ helicases in DNA inter-strand crosslink (ICL) repair and RecQ and Pif1
helicases in telomere maintenance. To perform this work, we will employ a variety of classic and cutting edge
experimental techniques, from standard in vitro enzymatic assays and model organism genetics to next-
generation sequencing, crosslinking mass spectrometry, and the development of custom click chemistry
probes. Overall, this work will provide fundamental data critical to understanding how PIF1 and RecQ family
helicases aid in the maintenance of genome stability, and it will ultimately lead to therapeutic targets and
treatments for helicase-linked diseases.
 I am requesting funds to purchase an Odyssey CLx Imaging System with a D-Digit attachment. This
imager enables two-channel (700 and 800 nm) near-infrared fluorescence and chemiluminescent imaging of
membranes, gels, plates, slides, and even large samples such as organs. The D-Digit further extends
functionality to the scanning, imaging, and analysis of agarose DNA gels stained with ethidium bromide or a
variety of safe stains (e.g., GelRed) without the need for ultraviolet transillumination. We routinely perform gel-
based assays to assess protein expression, protein-protein interactions, protein-nucleic acid interactions, and
the biochemical activities of DNA helicases and associated proteins, often using expensive radioisotopes for
labeling. Further, multiple pieces of shared equipment spread across buildings on campus are necessary to
visualize and analyze our experiments, and our time on the equipment is limited. The purchase of the Odyssey
CLx and D-Digit will provide a dedicated, isotope-free solution to our gel-based imaging and analysis needs.

## Key facts

- **NIH application ID:** 10388769
- **Project number:** 3R35GM133437-03S1
- **Recipient organization:** TRUSTEES OF INDIANA UNIVERSITY
- **Principal Investigator:** Matthew Linne Bochman
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $59,139
- **Award type:** 3
- **Project period:** 2019-08-01 → 2024-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10388769

## Citation

> US National Institutes of Health, RePORTER application 10388769, DNA helicases and associated factors in genome stability (3R35GM133437-03S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10388769. Licensed CC0.

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