# Molecular mechanisms that regulate polarity and spindle orientation in animals

> **NIH NIH R35** · UNIVERSITY OF OREGON · 2021 · $58,098

## Abstract

Project abstract
Funding is requested for the purchase of an AKTA pure 25 protein purification system for
purifying the reagents necessary for examining the biochemical activity of the Par complex, a
key aspect of the parent grant, R35GM127092. To reconstitute and interrogate Par complex
proteins to understand the mechanism by which polarity is regulated, highly pure reagents are
required. This is challenging for two reasons. First, the Par complex itself is difficult to obtain as
it is obtained from transiently transfected cultured cells. Second, our work requires making
various modified forms of Par complex proteins (e.g. truncations) and this instrument will allow
us to separate them from impurities with high resolution. We expect that the additional
capabilities provided by this instrument will have a significant and sustained positive impact on
our research program by increasing the reliability and reproducibility of key reagents.

## Key facts

- **NIH application ID:** 10388790
- **Project number:** 3R35GM127092-04S1
- **Recipient organization:** UNIVERSITY OF OREGON
- **Principal Investigator:** Kenneth E Prehoda
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $58,098
- **Award type:** 3
- **Project period:** 2018-05-01 → 2023-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10388790

## Citation

> US National Institutes of Health, RePORTER application 10388790, Molecular mechanisms that regulate polarity and spindle orientation in animals (3R35GM127092-04S1). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10388790. Licensed CC0.

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