# Administrative Supplement: Dynamics and evolution of synthetic and natural gene regulatory networks

> **NIH NIH R35** · STATE UNIVERSITY NEW YORK STONY BROOK · 2021 · $232,900

## Abstract

Project Summary: Administrative Supplement to NIGMS MIRA R35GM122561
Parent project title: Dynamics and evolution of synthetic and natural gene regulatory networks
This Administrative Supplement is based on NOT-GM-21-030, “Notice of Special Interest (NOSI):
Administrative Supplements for Equipment Purchases for NIGMS R01, R35, and R37 Awardees”. It is
a request for partial support of a FluidFM Bio system by Cytosurge AG, a Swiss Biotech company.
The FluidFM system has unique capabilities for repeated, single live cell-specific, direct intra-
cytoplasmic or intra-nuclear delivery and extraction of fluids, as well as nondestructive single cell
manipulation. These capabilities will be transformative in promoting progress with the parent grant,
which aims to learn how gene network dynamics and stochasticity affect single cells and thereby cell
populations. To achieve this, we proposed using synthetic gene networks to generate specific gene
expression patterns in space and time that serve as signals for natural gene networks, studying the
subsequent effects on cell population behavior and evolution by computational modeling and
experimental evolution. The FluidFM system will accelerate the stable genomic insertion of synthetic
gene circuits by coupled intranuclear delivery of CRISPR, recombinase and genetic payloads into
single cells, by cell isolation for clonal outgrowth, and by repeated “biopsies” taken from engineered
single living cells to study how their transcriptomic and proteomic networks respond to perturbations
by synthetic gene circuits. Overall, the FluidFM system will provide unique capabilities to illuminate
how complex networks enable control across scales of space and time in biology, from molecules to
cells. Addressing these questions will teach us how to control adapting cell populations, which is
relevant for understanding, predicting and possibly preventing cancer and microbial drug resistance.

## Key facts

- **NIH application ID:** 10388886
- **Project number:** 3R35GM122561-06S1
- **Recipient organization:** STATE UNIVERSITY NEW YORK STONY BROOK
- **Principal Investigator:** Gabor Balazsi
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $232,900
- **Award type:** 3
- **Project period:** 2017-04-01 → 2026-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10388886

## Citation

> US National Institutes of Health, RePORTER application 10388886, Administrative Supplement: Dynamics and evolution of synthetic and natural gene regulatory networks (3R35GM122561-06S1). Retrieved via AI Analytics 2026-06-04 from https://api.ai-analytics.org/grant/nih/10388886. Licensed CC0.

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