# Structure, Mechanism and Interactions of Type IA Topoisomerases

> **NIH NIH R35** · FLORIDA INTERNATIONAL UNIVERSITY · 2021 · $60,275

## Abstract

Project Summary PI: Yuk-Ching Tse-Dinh
This equipment supplement application requests funds to purchase an AKTA FPLC protein
purification system for the large scale purification of recombinant topoisomerase proteins that
are needed for obtaining new structures of topoisomerases by X-ray crystallography, and
biophysical studies to elucidate topoisomerase complex conformations, protein-protein
interactions and ligand interaction dynamics under the aims of the PI’s NIGMS active grants
R01-GM054226 and R35-GM139817. The AKTA FPLC protein purification system currently
available in the PI’s lab requires frequent repairs and needs to be replaced to avoid extensive
down times. The replacement of the AKTA FPLC purification system would allow us to produce
quickly and efficiently without bottlenecks milligram quantities of wild-type and mutant forms of
Escherichia coli and Mycobacterium tuberculosis topoisomerase I, E. coli topoisomerase III, and
human topoisomerase IIIB for structural and biophysical studies of their action on both DNA and
RNA substrates, as well as inhibitors. The proteins need to be produced in high purity for
studies in our lab at FIU, and are also needed by our collaborators at Argonne National Lab and
NIH for crystallization screens and single-molecule studies.

## Key facts

- **NIH application ID:** 10389425
- **Project number:** 3R35GM139817-01S1
- **Recipient organization:** FLORIDA INTERNATIONAL UNIVERSITY
- **Principal Investigator:** Yuk-Ching Tse-Dinh
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $60,275
- **Award type:** 3
- **Project period:** 2021-02-01 → 2026-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10389425

## Citation

> US National Institutes of Health, RePORTER application 10389425, Structure, Mechanism and Interactions of Type IA Topoisomerases (3R35GM139817-01S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10389425. Licensed CC0.

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