# Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms

> **NIH NIH R01** · FLORIDA INTERNATIONAL UNIVERSITY · 2021 · $126,472

## Abstract

Project Summary PI: Francisco Fernandez-Lima
This equipment supplement application requests funds to purchase a LEAP HDX Base System,
and corresponding HDExaminer Data Analysis Software to support instrumental advances in
tandem ion mobility – mass spectrometry (IMS/IMS-MS/MS) with the goal of providing needed
complementary structural information to the middle-down and top-down strategies developed for
the structural characterization of DNA binding proteins and ligand interaction dynamics under
the aims of the PI’s NIGMS active grant R01-GM134247. This equipment supplement request
will also streamline the application of solution HDX technology in tandem with unique middle-
down and top-down IMS- MS strategies for several active-funded NIGMS investigators at FIU
via the Advance Mass Spectrometry Facility, directed by the PI.

## Key facts

- **NIH application ID:** 10389482
- **Project number:** 3R01GM134247-03S1
- **Recipient organization:** FLORIDA INTERNATIONAL UNIVERSITY
- **Principal Investigator:** Francisco Fernandez-Lima
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $126,472
- **Award type:** 3
- **Project period:** 2019-09-20 → 2023-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10389482

## Citation

> US National Institutes of Health, RePORTER application 10389482, Development of Multidimensional, Linear and Differential Ion Mobility MS Separations for Middle-Down Proteoforms (3R01GM134247-03S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10389482. Licensed CC0.

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