# Deciphering Wnt-Ror signaling in cytoskeletal regulation and tissue shape control

> **NIH NIH R35** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2021 · $60,340

## Abstract

Supplement Summary
I am requesting an administrative supplement for the purchase of a BD Accuri C6 Plus Flow Cytometer. This
cytometer is equipped with two lasers and an automatic sampler capable of handling 24 samples at once. This
equipment will be crucial for completing the goals of my NIGMS R35 grant (R35GM119574) by enhancing the
throughput of a novel reporter assay that our lab developed, under the auspices of this R35 grant, to investigate
the biochemical mechanisms of the Wnt5a-Ror morphogenetic pathway. By coupling this reporter assays with a
newly optimized gene-replacement paradigm, we have begun to analyze a large number of mutant proteins
associated with dysfunction of the pathway and Robinow syndrome pathogenesis. Our workflow has now
exceeded the capacities of the currently available flow cytometers which can no longer handle the volume of
analyses efficiently, hence the new need for an automated system. The Department of Cell Biology and Human
Anatomy at UC Davis School of Medicine is committed to provide space to house the cytometer and funds for
instrument service. In summary, the requested flow cytometer will greatly accelerate the NIGMS-funded projects
in my lab, as well as those of other NIH investigators in the department and the greater UC Davis community.

## Key facts

- **NIH application ID:** 10389593
- **Project number:** 3R35GM119574-05S1
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** Hsin-Yi Henry Ho
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $60,340
- **Award type:** 3
- **Project period:** 2016-08-01 → 2022-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10389593

## Citation

> US National Institutes of Health, RePORTER application 10389593, Deciphering Wnt-Ror signaling in cytoskeletal regulation and tissue shape control (3R35GM119574-05S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10389593. Licensed CC0.

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