# Establishment of Active Chromatin Domains

> **NIH NIH R35** · BROWN UNIVERSITY · 2021 · $8,441

## Abstract

Project Summary: The overall goals of the MIRA are to define how domains of coordinate
gene activation are defined using Drosophila as a model system. We have identified an
essential transcription factor which is key to this process (CLAMP) and undergraduate student
Annie Huang will perform key experiments defining the genomic localization and function of
truncation mutants in the clamp gene. Annie will use microscopy to localize the mutants on
polytene chromosomes using available reagents and protocols and mRNA-seq to define their
function. Annie will have the opportunity and learn RNA-seq analysis using a new pipeline
which my lab recently developed called X vs. Y. Annie has not had many wet bench
opportunities due to Covid and has been taking computational courses online to prepare for her
summer research. Annie will make an important contribution to our understanding of the
coordinate regulation of gene activation which is a central goal of the current award.

## Key facts

- **NIH application ID:** 10391606
- **Project number:** 3R35GM126994-04S1
- **Recipient organization:** BROWN UNIVERSITY
- **Principal Investigator:** Erica Nicole Larschan
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $8,441
- **Award type:** 3
- **Project period:** 2018-04-01 → 2023-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10391606

## Citation

> US National Institutes of Health, RePORTER application 10391606, Establishment of Active Chromatin Domains (3R35GM126994-04S1). Retrieved via AI Analytics 2026-05-28 from https://api.ai-analytics.org/grant/nih/10391606. Licensed CC0.

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