# Quantitative 3D imaging of in situ nanoparticle movement and cellular behavior during neuroinflammation

> **NIH NIH R35** · UNIVERSITY OF WASHINGTON · 2021 · $28,536

## Abstract

PROJECT SUMMARY
Currently, the means to gather real-time molecular information from the diseased human brain is limited, and
high-throughput platforms that can assay neurological disease severity representative of the in vivo environment
are still lacking. This proposal reflects a foundational effort in my lab to generate a high throughout, quantitative,
real-time method of imaging cell and nanoparticle behavior, in 3-dimensions, within the neonatal or perinatal
brain in the presence of disease. We will specifically focus on neuroinflammation as a common disease hallmark
and use a transgenic rat model of autism. This approach will open up new avenues of research in the life sciences
and clinical sciences, by providing a platform for assessment of processes that are currently inaccessible to high-
throughput study, including developmental processes (i.e. synaptogenesis) and normal regulation of fluid flow
(i.e. regulation of waste removal via the glymphatic system). In addition, tracking and modelling nanoparticle co-
localization in, or interaction with, cells following injury could also provide new potential therapeutic targets.
Developing therapeutic technologies by leveraging their in vivo interactions with common disease hallmarks can
lead to more efficient translation of therapies across diseases with shared pathophysiological features. Given
that inflammation is a common factor across many central nervous system (CNS) diseases, results are expected
to provide insights and translate from the autism model used in this proposal to other models, including adult, of
brain disease.

## Key facts

- **NIH application ID:** 10392274
- **Project number:** 3R35GM124677-05S1
- **Recipient organization:** UNIVERSITY OF WASHINGTON
- **Principal Investigator:** Elizabeth A Nance
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $28,536
- **Award type:** 3
- **Project period:** 2017-08-01 → 2023-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10392274

## Citation

> US National Institutes of Health, RePORTER application 10392274, Quantitative 3D imaging of in situ nanoparticle movement and cellular behavior during neuroinflammation (3R35GM124677-05S1). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10392274. Licensed CC0.

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