# Core B - Advance Imaging Core

> **NIH NIH P01** · ALBERT EINSTEIN COLLEGE OF MEDICINE · 2022 · $293,909

## Abstract

Summary 
 The Proteostasis Advanced Imaging Core (Core B) referred in the Program Project (PP) text as “Image 
Core” will serve the four projects and the Innovation component of Core D of this PP. 
 The long-term goal is to provide support on image-based studies to understand the cellular and subcellular 
changes in autophagy dynamics during aging and in the context of Alzheimer disease (AD)-related proteotoxicity. 
 The specific aims of the core are: 1) to provide an imaging resource customized for the study of proteostasis 
in aging that will assist the PP investigators in the experimental preparation related to: basic microscopy 
techniques (wide-field microscopy, confocal microscopy, live cell imaging - at Mount Sinai/Einstein) and electron 
microscopy (at Einstein); 2) to provide a state-of-the art in vivo imaging platform based on two-photon microscopy 
(Innovation Unit - Mount Sinai) for dynamic analysis of changes in autophagy in brain and peripheral tissues in 
physiological aging and in disease; 3) to implement clearing imaging techniques and light-sheet microscopy for 
volume imaging to study autophagy in whole organs (Innovation Unit); 4) to develop STED superresolution 
imaging methods to study the dynamics of autophagy at the organelle level by nanoscale imaging (Innovation 
Unit). 
 Components: 1) The Imaging unit provides advice on sample preparation/processing, techniques and 
procedures for imaging in vivo and fixed samples. Performs electron microscopy (at Einstein) and specialized 
imaging procedures (at Mount Sinai) such as intravital microscopy, light-sheet imaging, STED superresolution 
and assists with imaging data interpretation; 2) The innovation unit (at Mount Sinai) will develop and implement 
procedures specific for analysis of autophagy at the tissue level (light-sheet imaging, intravital microscopy) and 
at the single molecule level (superresolution imaging) in aging and AD. 
 Services: The core will continue offering i) assistance with regular wide-field microscopy, real-time live cell 
imaging, confocal and electron microscopy, ii) distributing common reagents (antibodies, probes and 
fluorescence dyes), iii) sharing detailed protocols for image-based procedures and iv) compiling the image- 
based information for the PP data base. In addition, during this period the core has now incorporated: i) high- 
resolution imaging procedures such as intravital two-photon microscopy, superresolution STED imaging and 
light-sheet microscopy for volume imaging, ii) assistance with sample preparation for these procedures (in vivo 
mouse preparation, clearing methods) and iii) access to new image analysis software (IMARIS, ARIVIS, 
Huygens) available at the Microscopy CoRE at Mount Sinai. 
Relevance: The new technology incorporated in the core now allows dynamic and molecular resolution imaging 
to study aging and AD-related changes in autophagy at the subcellular level. This information is essential for 
future implementation o...

## Key facts

- **NIH application ID:** 10397005
- **Project number:** 5P01AG031782-15
- **Recipient organization:** ALBERT EINSTEIN COLLEGE OF MEDICINE
- **Principal Investigator:** Jose Javier Bravo-Cordero
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $293,909
- **Award type:** 5
- **Project period:** 2009-02-15 → 2025-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10397005

## Citation

> US National Institutes of Health, RePORTER application 10397005, Core B - Advance Imaging Core (5P01AG031782-15). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10397005. Licensed CC0.

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