# Service Centers for Cryo-Electron Tomography

> **NIH NIH U24** · STANFORD UNIVERSITY · 2021 · $435,366

## Abstract

Project Summary
This is a supplement request for purchasing two instruments. One is a high-pressure freezing apparatus
which is needed to preserve the cell or tissue in vitrified state for cryoFIB and cryoET experiments. This
approach is well documented in the literature to be effective. The proposed Leica ICE+ Pearl have an
added advantage that we can manipulate the sample with external electrical or optical stimuli
immediately before freezing. This will allow us to subject the cell sample in different physical conditions
mimicking physiological conditions after which the sample will be frozen to capture its structure for
cryoET observation. The second instrument is a micropatterning device to write on the EM grid to make
the cells spreading more readily across the grid square rather than the grid bars. Our preliminary
experiments demonstrate the effectiveness of this approach.

## Key facts

- **NIH application ID:** 10397372
- **Project number:** 3U24GM139166-02S1
- **Recipient organization:** STANFORD UNIVERSITY
- **Principal Investigator:** Wah Chiu
- **Activity code:** U24 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $435,366
- **Award type:** 3
- **Project period:** 2020-09-16 → 2024-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10397372

## Citation

> US National Institutes of Health, RePORTER application 10397372, Service Centers for Cryo-Electron Tomography (3U24GM139166-02S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10397372. Licensed CC0.

---

*[NIH grants dataset](/datasets/nih-grants) · CC0 1.0*