# Core B:  Animal, Cell and Tissue Culture Core

> **NIH NIH P01** · UNIVERSITY OF ROCHESTER · 2022 · $455,588

## Abstract

SUMMARY: The Animal, Cell and Tissue Culture Core (Core B) is responsible for providing investigators of the
Program Project (PPG) with animal models, tissues and cells necessary to achieve their specific aims.
Experiments of this PPG focus on identifying mechanisms responsible for the differences in longevity between
species of rodents. Rodents offer a unique opportunity for such comparative study of longevity because of the
over 10-fold differences in lifespans represented within a single mammalian order. For example, short-lived
rodents, such as the mouse and rat, live 3-5 years, while long-lived rodents, such as the naked mole rat, blind
mole rat, grey squirrel, beaver, and porcupine have maximum lifespans ranging from 21 to 32 years. To enable
the study of molecular mechanisms of longevity in these animals, it is essential to provide access to biological
materials from several individuals of multiple rodent species. Core B maintains and replenishes, as needed, the
collection of tissues and primary cells from over 22 species of rodents. The Core provides project investigators
with tissues, cells, DNA and RNA, and performs stress treatments on animals. In addition, Core B will maintain
mice, naked mole rats, blind mole rat and Damaraland mole rat colonies for the use of project investigators.
Considering the effort needed to collect and establish cell cultures from non-standard organisms that are not
commercially available, this centralized resource saves time and effort, and reduces costs. Core B will generate
and breed mouse models with longevity interventions engineered based on the longevity mechanisms
discovered by project investigators in long-lived rodent species. This includes mice overexpressing naked mole
rat hyaluronan synthase 2 gene (nmrHAS2 mice), beaverized SIRT6 mice, and mice treated with hyaluronidase
inhibitors. Core B will perform longevity studies with these mice and analyze the effect of interventions on health.
Maintaining the centralized rodent collection standardizes culture conditions, quality control, and biological
samples for use across PPG projects, improves reproducibility of results, and allows the analysis of the same
individual animals by several assays and projects minimizing animal use.

## Key facts

- **NIH application ID:** 10399518
- **Project number:** 5P01AG047200-09
- **Recipient organization:** UNIVERSITY OF ROCHESTER
- **Principal Investigator:** Andrei Seluanov
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $455,588
- **Award type:** 5
- **Project period:** 2014-05-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10399518

## Citation

> US National Institutes of Health, RePORTER application 10399518, Core B:  Animal, Cell and Tissue Culture Core (5P01AG047200-09). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10399518. Licensed CC0.

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