# Imaging Core

> **NIH NIH P01** · OHIO STATE UNIVERSITY · 2022 · $217,509

## Abstract

Core C: Abstract 
The overarching theme of program is to dissect a new concept that in Acute Respiratory 
Distress Syndrome (ARDS), immunosuppression is a signature manifestation secondary to 
unique, combinatorial pathways that modulate epithelial and myeloid cell viability and innate 
immune function. We focus on pneumonia as a conceptual framework to study 
immunosuppression, as pneumonia is a common cause and risk factor for ARDS. The faculty 
within the Imaging Core have been collaborating continuously and productively with the PI’s of 
this program for 23 years in various aspects of immunobiology, lipid biology 
andairway/pulmonary biology resulting in 37 collaborative papers. 
 The principal roles of the imaging core will be; careful analysis of cellular and molecular 
processes associated with apoptosis, necrosis, ferroptosis and other cell death mechanisms as 
needed, characterization and quantification of cell types within tissues, examination of protein 
expression during cell death, examination of cell-cell interactions in vitro, quantitative analysis 
of cellular migration in vivo and ex vivo individual cells within tissues, and quantitative 
measurement of the levels and patterns of expression of chemokines and cell-type markers 
various samples at all levels of resolution. These studies will employ the full array of current 
light, and potentially, electron microscopic methods including: single and multicolor 
fluorescence microscopy, laser confocal microscopy live cell imaging, transmission electron 
microscopy and computer-aided morphometric analyses . The Center for Biologic Imaging, in 
which this core service will be performed, is designed for the purpose of providing state of the 
art microscopic technologies to its users. It is equipped to perform a continuum of optical 
methods including all types of light and electron microscopy essential to this Program Project.

## Key facts

- **NIH application ID:** 10399558
- **Project number:** 5P01HL114453-09
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** SIMON C WATKINS
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $217,509
- **Award type:** 5
- **Project period:** 2014-01-03 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10399558

## Citation

> US National Institutes of Health, RePORTER application 10399558, Imaging Core (5P01HL114453-09). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10399558. Licensed CC0.

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