# Consortium for large-scale production and phenotyping of knockout mice (UM1)

> **NIH NIH UM1** · BAYLOR COLLEGE OF MEDICINE · 2021 · $2,559,904

## Abstract

ABSTRACT
The long-term goal of the International Mouse Phenotyping Program (IMPC) and Knockout Mouse Phenotyping
Project (KOMP2) is to develop a resource of targeted mutations in mice for every protein-coding gene in the
mammalian genome that the research community can use to elucidate gene function in human biology and
disease. In Phase 2 of funding (2017-2021), the Baylor College of Medicine-Harwell (BasH) consortium was
tasked with the generation of 1000 mutant mouse lines using CRISPR/Cas9 technology, phenotyping of these
null allele lines, quality control assessment of genotype and phenotype data, cryopreservation of mutant strain
germplasm, and providing mice and data to the research community. However, due to the outbreak of emerging
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) and the resulting reduction
of research activities at our institutes, BasH will not have completed phenotyping, data quality control
assessment, and cryopreservation and distribution of all 1000 mutant lines. To achieve our goals, the project
timeline needs to be adjusted and additional budgetary support provided. This supplement requests an additional
year of funding to complete phenotyping of 1000 mutant mouse lines, conduct quality control assessment of
data, cryopreservation of germplasm, and distribution of mice and data to the research community. We will
perform broad-based adult phenotyping on all mutant lines. We will also assess homozygous lethal and
subviable lines in an embryonic phenotyping pipeline. All allele and phenotype data will be submitted in real time
to the Data Coordination Center, ensuring that BasH data is disseminated to the wider biomedical scientific
community. Cryopreserve germplasm will be distributed to the Mutant Mouse Resource and Research Centers
at the University of North Carolina and University of Missouri. Finally, we will use CRISPR genome editing
technology to produce 20 new null allele lines to prepare for the next phase of KOMP2 funding.

## Key facts

- **NIH application ID:** 10399851
- **Project number:** 3UM1HG006348-10S3
- **Recipient organization:** BAYLOR COLLEGE OF MEDICINE
- **Principal Investigator:** Mary E Dickinson
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $2,559,904
- **Award type:** 3
- **Project period:** 2011-09-28 → 2022-08-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10399851

## Citation

> US National Institutes of Health, RePORTER application 10399851, Consortium for large-scale production and phenotyping of knockout mice (UM1) (3UM1HG006348-10S3). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10399851. Licensed CC0.

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