# METABOLIC ANALYSIS INSTRUMENTATION TO ENHANCE TARGETED THERAPEUTICS STUDIES

> **NIH NIH P20** · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · 2021 · $249,035

## Abstract

The COBRE Center for Targeted Therapeutics (CTT) at the University of South Carolina (UofSC) supports
research that seeks to develop new and more effective classes of drugs against various diseases, by aiming
these drugs at molecular and cellular targets that play a key role in the disease. Many therapeutic targets in
different diseases either regulate or are functionally impacted by cellular metabolism. The ability to interrogate
the metabolic effects of genetic or pharmacological modulation of such targets is critical to many CTT-affiliated
investigators. The purpose of this proposal is to acquire an Agilent Seahorse Extracellular Flux (XF) Analyzer,
including a module to facilitate data imaging and normalization. This will replace an older version of this
instrument that cannot perform the high content imaging analyses and is no longer supported by the
manufacturer. This instrument will belong to the Microscopy and Flow Cytometry Core of the CTT and will be
overseen by a highly experienced investigator in cellular metabolic studies. There are several laboratories,
including former and present CTT-funded investigators, which will be able to use the metabolic analysis
instrument to increase their ability to achieve their research goals. Some of the projects by CTT-affiliated
investigators that have an immediate need for the Seahorse Analyzer to investigate (i) the impact of metabolite
derived protein modifications on neurodegenerative diseases (ii) metabolic effects of HIV-related changes in
brain microglia, (iii) metabolic analysis of age-related changes in neurotransmission, (iii) effects of transcriptional
Mediator kinase on cellular metabolism, (iv) cellular metabolic effects of nanoparticle and scaffold implants that
deliver therapeutics to support tissue regeneration. Acquisition of this instrument will allow the continuation of
these targeted therapeutics projects and will offer a more reliable way to use the data acquired. The capacity to
measure cellular metabolism responses and adaptations will enhance the current CTT-supported projects and
will allow CTT to extend its umbrella to other targeted therapeutics researchers whose research areas
incorporate metabolic analyses.

## Key facts

- **NIH application ID:** 10400414
- **Project number:** 3P20GM109091-08S2
- **Recipient organization:** UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
- **Principal Investigator:** IGOR B RONINSON
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $249,035
- **Award type:** 3
- **Project period:** 2014-07-10 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10400414

## Citation

> US National Institutes of Health, RePORTER application 10400414, METABOLIC ANALYSIS INSTRUMENTATION TO ENHANCE TARGETED THERAPEUTICS STUDIES (3P20GM109091-08S2). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10400414. Licensed CC0.

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