# Resource for native mass spectrometry guided structural biology-Refeyn OneMP Mass Photometer

> **NIH NIH P41** · OHIO STATE UNIVERSITY · 2021 · $136,002

## Abstract

The P41 Resource for Native Mass Spectrometry Guided Structural Biology (nMS->SB Resource),
established in 2018, is a national Biomedical Technology Research Resource that seeks to develop and
disseminate novel research technologies and methods for structural biology. The Resource is developing and
disseminating a suite of technologies under five Technology Research and Development (TR&D) projects; these
include (TR&D 1-3) novel surface-induced dissociation (SID) cells coupled to high-resolution ion mobility (IM)
and high-resolution mass spectrometry (HRMS), (TR&D 4) online purification and separation schemes, and
(TR&D 5) computational tools for structure determination from experimental nMS data. The Resource has
successfully supported the efforts of numerous biomedical researchers across the country and the globe to
characterize a variety of noncovalent protein and RNA/DNA:protein complexes, including synthetic amyloid beta
(Aβ) and tau aggregates, novel de novo designed protein complexes from the Baker laboratory (University of
Washington), orthologous 20S proteasomes expressed by the Sharon laboratory (Weizmann Institute) and
recently Ebola virus matrix protein VP40 (La Jolla Institute of Immunology). Our protein:protein and
nucleoprotein complexes are typically run at mass spectrometry-friendly concentrations and in mass
spectrometry-compatible buffers/ electrolytes. Acquisition of an instrument (Refeyn OneMP Mass Photometer)
for light-based mass photometry (interferometry) measurements will provide new technology for our DBP and
C&S investigators, allowing us to assess small sample volumes at very low concentrations (pM to low nM). Mass
photometry (MP) will provide a quick screen of sample quality and oligomeric distributions present in the sample
in the investigator’s provided storage buffer, allowing MS team members to decide on the appropriate next steps
for the sample. We will determine whether the sample will go back to the investigator for improvements or
whether it is ready for higher resolution, higher mass accuracy native mass spectrometry measurements. For a
wide variety of protein complex types, provided by a broad set of DBP and C&S projects, we will establish
complex types and concentration behaviors for which the MP and nMS results are duplicative and those for
which the two sets of results provide additive/complementary data to characterize the complex more fully over a
large range of conditions (oligomerization time/kinetics, concentrations, buffer conditions).

## Key facts

- **NIH application ID:** 10400442
- **Project number:** 3P41GM128577-04S1
- **Recipient organization:** OHIO STATE UNIVERSITY
- **Principal Investigator:** Vicki H. Wysocki
- **Activity code:** P41 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $136,002
- **Award type:** 3
- **Project period:** 2018-07-01 → 2023-06-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10400442

## Citation

> US National Institutes of Health, RePORTER application 10400442, Resource for native mass spectrometry guided structural biology-Refeyn OneMP Mass Photometer (3P41GM128577-04S1). Retrieved via AI Analytics 2026-05-26 from https://api.ai-analytics.org/grant/nih/10400442. Licensed CC0.

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