# Shared Resource - Microscopy & Imaging Shared Resource

> **NIH NIH P30** · GEORGETOWN UNIVERSITY · 2022 · $58,810

## Abstract

The Microscopy & Imaging Shared Resource (MISR) was established in 1989 to provide 1) access to an array of
well-maintained instrumentation for Georgetown Lombardi Comprehensive Cancer Center (LCCC) investigators
wishing to apply light microscopy and image analysis to cancer research and 2) the expertise, training and reagents
necessary for investigators to make optimal use of the instruments available. The MISR provides instrumentation
for bright-field microscopy, routine fluorescence microscopy and more advanced applications, including confocal
microscopy, microinjection, total internal reflection microscopy (TIRF), multispectral emission imaging,
fluorescence lifetime imaging, Förster resonance energy transfer (FRET), fluorescence recovery after
photobleaching (FRAP) and deep tissue multiphoton imaging. Live cell, time-lapse imaging is possible using five
different wide-field and confocal systems. Using MetaMorph v7.8.13 offline image analysis and Volicity v6.3.0 3D
Image Analysis Software, MISR develops automated routines for users carrying out common assays, such as cell
tracking, proliferation, studies of mitosis and apoptosis plus angiogenesis experiments. The MISR also supports
cytogenetics and molecular cytogenetics services and provides the following:
• Access to nine major digital microscope workstations, transmission electron microscope and stand-alone
 image analysis workstations. It also supports 16 image analysis software licenses in individual laboratories.
• Expert advice, training and assistance with sophisticated microscopy and image analysis approaches in
 cancer research.
Since the last submission, the MISR 1) replaced an aging confocal system with a new highly capable Leica SP8
AOBS laser scanning confocal microscope, 2) upgraded the TIRF system with a new camera and a coded stage
that extends the imaging modalities available and 3) currently supports multiple high-impact studies
demonstrating the interaction between distinct cell surface molecules in tumorigenesis and inflammation. The
MISR Director, M. Johnson, PhD, leads a team with a wealth of expertise in light and electron microscopy plus
image analysis. In 2017, 20 LCCC members representing all four Research Programs (Breast Cancer [BC],
Cancer Prevention and Control [CPC], Experimental Therapeutics [ET], and Molecular Oncology [MO]) used the
MISR.

## Key facts

- **NIH application ID:** 10400651
- **Project number:** 5P30CA051008-29
- **Recipient organization:** GEORGETOWN UNIVERSITY
- **Principal Investigator:** MICHAEL D JOHNSON
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $58,810
- **Award type:** 5
- **Project period:** 1997-08-15 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10400651

## Citation

> US National Institutes of Health, RePORTER application 10400651, Shared Resource - Microscopy & Imaging Shared Resource (5P30CA051008-29). Retrieved via AI Analytics 2026-05-25 from https://api.ai-analytics.org/grant/nih/10400651. Licensed CC0.

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