PROJECT SUMMARY/ABSTRACT Colony Genetics Core Genetic characterization of animals in the Yerkes specific pathogen free (SPF) breeding colony is conducted by the Colony Genetics Core. Our efforts are focused on preserving genetic diversity through breeding and identifying animals suitable for AIDS research assignment. The Colony Genetics Core recently transitioned most wet-bench workflows onto liquid-handling robots and switched to a sample labeling strategy that facilitates realtime tracking and automation. We developed and maintain several purpose-built tools that comprise our analytical framework for defining genetic parentage, calculating genetic value, and developing breeding recommendations. We make these tools, and all genetics datasets and analyses, available to the YNPRC community and others through a web-based resource that we developed, Colony Informatics. Across three Specific Aims, we will continue to ensure that animals are expeditiously genotyped for AIDS-related assignment and to prioritize animals for retention as breeders. We will also begin to incorporate comprehensive genetic characterization of breeders through exome sequencing. Altogether, our efforts are highly collaborative and integrative; we routinely work with the other SPF cores, veterinarians, and investigators to ensure that the needs of the colony are readily met. Specific Aim 1: Provide breeding recommendations based on parentage and genetic value genotyping. Specific Aim 2: Perform allele-specific genotyping for AIDS research assignment. Specific Aim 3: Perform primatized exome sequencing of new breeders to surveil for new alleles. Taken together, these proposed aims will ensure the genetic diversity and sustainability of the Yerkes SPF colony for years to come.