# Skeletal Muscle Stem Cells Derived from Teratomas

> **NIH NIH R01** · UNIVERSITY OF MINNESOTA · 2022 · $426,186

## Abstract

Abstract
Pluripotent stem cells hold tremendous promise for the eventual development of cell therapies for muscle
disease; however it has proven exceptionally difficult to derive cells with meaningful in vivo regeneration
potential from unmodified wild type (WT) pluripotent stem cells. Mouse transplantation studies with WT PSC-
derived cells are rare, and success in the field currently equates to generation of a few hundred fibers in a
transplanted tibialis anterior muscle with 3-4,000 total fibers. We have recently discovered that when mouse
PSCs are differentiated into teratomas in vivo, they generate large numbers of skeletal muscle stem cells, and
these cells have tremendous in vivo functional potential, regenerating the great majority of the TA muscle with
force-generating muscle fibers upon transplantation. We propose a series of studies aimed at understanding
how these cells arise, what makes them unique, translating these findings to the human system, and using this
knowledge to improve in vitro differentiation protocols.

## Key facts

- **NIH application ID:** 10403612
- **Project number:** 5R01AR075413-04
- **Recipient organization:** UNIVERSITY OF MINNESOTA
- **Principal Investigator:** Michael Kyba
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $426,186
- **Award type:** 5
- **Project period:** 2019-05-01 → 2024-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10403612

## Citation

> US National Institutes of Health, RePORTER application 10403612, Skeletal Muscle Stem Cells Derived from Teratomas (5R01AR075413-04). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10403612. Licensed CC0.

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