# Enhancement of existing ABSL3 facilities to accommodate expanded mouse validation and phenotyping

> **NIH NIH U42** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2021 · $485,622

## Abstract

ABSTRACT & SCOPE OF WORK
The Mutant Mouse Resource and Research Center at the University of California, Davis (MMRRC-UC Davis)
is pleased to submit this administrative supplement for up to 1 year of support in response to ORIP’s
participation in PA-20-272, “Administrative Supplements to Existing NIH Grants and Cooperative Agreements”
specifically related to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19).
This application addresses a number of the stated objectives of the call to support general and biocontainment-
related alterations and renovations of existing facilities and to purchase equipment to facilitate COVID-19 and
other infectious disease research in small animals. Specifically, this application requests funds to enhance and
expand capacity and functionality of our existing ABSL3 vivarium and testing facility by 1) purchasing additional
mouse caging and related husbandry equipment to double our ABSL3 biosafety housing, maintenance, and
testing capacity for mice, 2) obtaining equipment that facilitates increased mouse handling and testing
throughput for infectious disease research requiring ABSL3 containment, and 3) procuring laboratory
instrumentation for virus preparation and trituration, and mouse challenge and phenotyping activities dedicated
to infectious disease research, including SARS-CoV-2 and COVID-19. Funding of this project will accomplish
3 things. First, it will enable us to conduct additional and more complex mouse-related studies than we can
currently perform simultaneously, thus accelerating and extending our abilities to respond to a greater variety
of researcher needs and in a more timely manner. Second, it will allow us to conduct long-term studies on
mouse models after infectious virus challenge, which will permit and catalyze research on aged mice and the
pathophysiological consequences and complications of virus infection on the host, such as post-acute
sequellae of COVID-19 (PASC). Third, it will permit efficiencies in virus challenge studies and consolidation of
phenotyping activities within the ABSL3 facility, such as measuring body weight kinetics, assessing cardiac
and lung function, performing necropsy and harvesting tissues (organs, cells, blood, intestinal contents, etc),
and whole animal imaging. Together, these upgrades will significantly enhance our ability to conduct infectious
disease research on mouse models that require ABSL3 conditions in support of the scientific community and
users of the MMRRC resource. This proposal directly addresses a number of the stated objectives of this
special NIH call. The parent peer-reviewed NIH funded grant (U42OD012210) has been competitively renewed
(as of January 1, 2020) for 5 years. A budget of $482,305 direct costs ($485,622 total costs) requested for the
remaining months of the current grant year (until January 31, 2022) is less than half the annual total direct cost
budget of the parent grant. If funded by this supplement, the ...

## Key facts

- **NIH application ID:** 10406101
- **Project number:** 3U42OD012210-22S2
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** KC KENT LLOYD
- **Activity code:** U42 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $485,622
- **Award type:** 3
- **Project period:** 1999-09-30 → 2025-01-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10406101

## Citation

> US National Institutes of Health, RePORTER application 10406101, Enhancement of existing ABSL3 facilities to accommodate expanded mouse validation and phenotyping (3U42OD012210-22S2). Retrieved via AI Analytics 2026-06-11 from https://api.ai-analytics.org/grant/nih/10406101. Licensed CC0.

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