# Genome Editing Core

> **NIH NIH P30** · JOSLIN DIABETES CENTER · 2022 · $242,656

## Abstract

GENOME EDITING CORE: Abstract
Investigating mechanisms that underlie diabetes and its complications requires relevant experimental models.
The Genome Editing Core (GEC) was established to assist investigators in generating cell and mouse models
that can accelerate the development of new approaches to prevention, treatment, and ultimately a cure for
diabetes. The GEC provides investigators the tools and expertise to leverage the latest CRISPR methodologies
for the generation of both mouse and cellular models. The first component of this core, implemented
approximately 5 years ago, is a mouse genome editing service. GEC staff assist investigators in the design and
generation of custom mouse models with gene insertions, deletions and modifications of many kinds. The GEC
is uniquely positioned to engineer the genome of the most relevant mouse models for diabetes, including the
nonobese diabetic mouse model for type 1 diabetes and strains used in type 2 diabetes research (e.g. C57BL6
and C57BLKS). These models enable investigators to evaluate the consequences of genome edits at the
systemic level in physiological conditions. The second component of this core enables powerful cell-based,
genome-scale forward genetic screens through a CRISPR screening service and represents a major core
evolution to meet the needs of the Joslin research base. GEC leadership brings outstanding expertise in this
approach, and over the past year the core has acquired several genome-scale mouse and human CRISPR
libraries and completed successful screens. In the present application, CRISPR screening services will be
offered to perform genome-wide screens in the many various cell types to uncover mechanisms and identify
novel targets in the pathogenesis of diabetes and its complications. In sum, the GEC provides a state-of-the art
platform for DRC investigators to manipulate the genome of mouse and human cells at a small scale for specific
hypothesis-driven projects and at a large scale for exploratory, hypothesis-generating research endeavors. The
GEC further allows investigators to test genome edits in mouse models to explore gene function at the systemic
level in physiological conditions.

## Key facts

- **NIH application ID:** 10407759
- **Project number:** 2P30DK036836-36
- **Recipient organization:** JOSLIN DIABETES CENTER
- **Principal Investigator:** Peng Yi
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $242,656
- **Award type:** 2
- **Project period:** 1997-02-15 → 2027-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10407759

## Citation

> US National Institutes of Health, RePORTER application 10407759, Genome Editing Core (2P30DK036836-36). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10407759. Licensed CC0.

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