# Flow Cytometry and Technology Development

> **NIH NIH P30** · JOHNS HOPKINS UNIVERSITY · 2022 · $288,205

## Abstract

PROJECT SUMMARY/ABSTRACT
 The Sidney Kimmel Comprehensive Cancer Center (SKCCC) Flow/Mass Cytometry Shared Resource
(SR) has continuously offered state-of-the-art cell sorting and flow analysis services to SKCCC Members from
its inception in 1980. In 2013, the Flow Cytometry SR merged with the Human Immunology (HI) SR, but HI has
since been more appropriately integrated with a newly established SR that handles clinical specimen
collections and processing for all SKCCC studies. To expand the capabilities for tumor microenvironment and
immune profiling, the SKCCC recently acquired mass cytometry technologies for suspension and imaging
analyses. These resources were integrated into the collective Flow/Mass Cytometry and Technology
Development (FCTD) Shared Resource (SR) in 2020. While many diverse therapies are being evaluated at the
SKCCC, many studies overlap in their requirement for flow/mass cytometry analysis and cell sorting of immune
cells. These services are provided in a timely, flexible and affordable manner to SKCCC Members from across
the medical campus. Services provided by the FCTD SR do not duplicate services available elsewhere, and
the SR currently operates the only mass cytometer at the institution. Cytometry services range from basic two-
color flow analyses to complex cell-sorting experiments involving up to 16 colors, as well as 20-plex mass
cytometry profiling based on 30-40+ parameters, plus training, data analysis and consultation services.
Successful execution of these services has led to: 1) a novel analytical pipeline for determining T cell repertoire
changes in response to immunotherapy regimens (i.e., the Mutation-Associated Neoantigen Functional
Expansion of Specific T Cells Assay), 2) synergistic incorporation of multimer-based methodologies for
investigating neoantigen-specific T cell subpopulations, and 3) a platform for innovative integration of
scRNAseq datasets to study T cell responses in collaboration with the SKCCC Experimental and
Computational Genomics SR. Moreover, the application of mass cytometry techniques, as currently offered by
the FCTD SR, has already enabled discovery for preclinical and translational researchers at the SKCCC.
Collectively, these resources significantly improve the ability to functionally characterize immune cells in cancer
therapy, vaccine trials and preclinical studies.
SKCCC Managed Shared Resource
Reporting Period: January 1, 2020 to December 31, 2020

## Key facts

- **NIH application ID:** 10409360
- **Project number:** 2P30CA006973-59
- **Recipient organization:** JOHNS HOPKINS UNIVERSITY
- **Principal Investigator:** VICTOR M LEMAS
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $288,205
- **Award type:** 2
- **Project period:** 1997-05-07 → 2027-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10409360

## Citation

> US National Institutes of Health, RePORTER application 10409360, Flow Cytometry and Technology Development (2P30CA006973-59). Retrieved via AI Analytics 2026-05-27 from https://api.ai-analytics.org/grant/nih/10409360. Licensed CC0.

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