# Clonal tracking and molecular characterization of hematopoiesis under stress

> **NIH NIH P01** · MASSACHUSETTS GENERAL HOSPITAL · 2021 · $50,400

## Abstract

RESEARCH SUMMARY
Hematopoiesis is now recognized as the integrated activity of multiple, largely progenitor clones with
heterogeneous behavior. Clonal complexity is thought to diminish with age and human studies suggest that
oligoclonal hematopoiesis with disease associated mutations is common. The presence of such clones incurs
a substantial risk of hematologic neoplasia and all cause mortality. The overall goal of this proposal is to
understand the molecular drivers of clonal behavior under stress conditions and to test whether modifying
those drivers is capable of changing the relative competitive balance of normal and abnormal, risk bearing
clones in animal models. We will use techniques of fluouresence clonal tracking in the mouse developed by us
and compare these with retrotransposon clonal marking techniques in the mouse of Carmago and
fluorescence clonal tracking techniques in the zebrafish developed by Zon. Further, we will characterize
genetic characteristics of clones at single cell resolution using techniques of Tenen and define ncRNA
candidate molecular drivers with Tenen. Finally, we will target epigenetic modifiers defined by Orkin, Tenen
and Zon to test the impact on clonal competition between normal and mutant clones in vivo. Combined these
complementary and collaborative approaches will point to methods for altering competitive relationships in
hematopoiesis in favor of normal cells.

## Key facts

- **NIH application ID:** 10413504
- **Project number:** 3P01HL131477-05S1
- **Recipient organization:** MASSACHUSETTS GENERAL HOSPITAL
- **Principal Investigator:** David T Scadden
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $50,400
- **Award type:** 3
- **Project period:** 2020-06-15 → 2023-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10413504

## Citation

> US National Institutes of Health, RePORTER application 10413504, Clonal tracking and molecular characterization of hematopoiesis under stress (3P01HL131477-05S1). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10413504. Licensed CC0.

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