# Project 3 - Characterizing the Amplification Factories of Epstein-Barr Virus and Kaposi's Sarcoma-associated Herpesvirus

> **NIH NIH P01** · UNIVERSITY OF WISCONSIN-MADISON · 2022 · $396,458

## Abstract

PROJECT 3 − SUMMARY/ABSTRACT
Epstein-Barr Virus (EBV) and Kaposi's Sarcoma Herpesvirus (KSHV) cause lymphomas, carcinomas, and
sarcomas in people across the world. No specific anti-viral therapies or vaccines are available now to treat
these tumors or to prevent infections with EBV or KSHV. During the current funding period, we have
developed new technologies and used them to uncover exciting discoveries about entry into and progression
through the productive or lytic phase of the life-cycle of EBV. We have developed live-cell imaging of intact,
infectious genomes of EBV and KSHV and found that during its lytic cycle EBV amplifies its DNA in discrete
sites or factories, mediates a dramatic concentration of cellular chromatin at the nuclear periphery, and inhibits
synthesis of histone chaperones. We have also found that this amplified viral DNA lacks histones and is the
template for viral late gene transcription. We shall examine at the single cell level the formation of both EBV's
and KSHV's amplification factories, their consolidation of cellular chromatin, and their mechanisms of
transcription of viral late genes in these factories. These detailed studies will identify virus-specific processes
that can be targeted to block virus spread.

## Key facts

- **NIH application ID:** 10414877
- **Project number:** 5P01CA022443-45
- **Recipient organization:** UNIVERSITY OF WISCONSIN-MADISON
- **Principal Investigator:** WILLIAM M. SUGDEN
- **Activity code:** P01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $396,458
- **Award type:** 5
- **Project period:** 1997-02-01 → 2024-02-29

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10414877

## Citation

> US National Institutes of Health, RePORTER application 10414877, Project 3 - Characterizing the Amplification Factories of Epstein-Barr Virus and Kaposi's Sarcoma-associated Herpesvirus (5P01CA022443-45). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10414877. Licensed CC0.

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