# Bridging the Glycome and Proteome with Chemical Biology

> **NIH NIH R35** · SCRIPPS RESEARCH INSTITUTE, THE · 2022 · $443,746

## Abstract

PROJECT SUMMARY
Glycoproteins and proteoglycans are important protein glycoconjugates in the cell. They can
control many biological events, such as growth factor signaling, cell adhesion, and differentiation,
to impact cancer biology, development, and immunity. While vital to human health and physiology,
we currently do not have a comprehensive molecular understanding of how these molecules
orchestrate their functions. Many techniques study these molecules in isolation – focusing on only
the protein or glycan component alone. This current approach severely underappreciates the
structural diversity of protein glycoconjugates, and it has left us with a narrowed understanding of
how protein glycoforms can differentially regulate biology. Furthermore, it has impeded pathways
to use glycoform-dependent interactions for drug discovery. Our research program integrates
techniques in chemical biology, molecular biology, and protein engineering, in order to address
these gaps. In this application, we describe our plans to create defined semi-synthetic
glycoproteins and proteoglycans, and to use these materials to study their interactions in live cells.
Identifying the glycoproteins associated with glycan-binding proteins allows the use of targeted
genetic approaches to study their functional contributions. The insights developed from these
studies will create an opportunity to discover small molecules that can selectively modulate
glycan-binding protein and glycoprotein interactions in order to regulate important biological
events.

## Key facts

- **NIH application ID:** 10415215
- **Project number:** 5R35GM142462-03
- **Recipient organization:** SCRIPPS RESEARCH INSTITUTE, THE
- **Principal Investigator:** Mia L Huang
- **Activity code:** R35 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $443,746
- **Award type:** 5
- **Project period:** 2021-06-01 → 2026-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10415215

## Citation

> US National Institutes of Health, RePORTER application 10415215, Bridging the Glycome and Proteome with Chemical Biology (5R35GM142462-03). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10415215. Licensed CC0.

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