# Immune Responses to Malaria, HIV and SARS-CoV-2 Infection and Immunization - Service Core

> **NIH NIH U19** · SEATTLE CHILDREN'S HOSPITAL · 2022 · $650,701

## Abstract

Abstract
The immune system is composed of many heterogeneous cell types that respond in a synergistic
manner to mount both immediate and long-lived immunity against pathogens. The complexity of
the immune system necessitates an integrated view of multiple biological modalities to understand
different roles of varying cell types of the immune system. Detailed transcriptomic and protein
analysis at the single cell level is essential to follow the immune system’s response to pathogens
in mounting an antigen specific response. With heavy emphasis on assays that facilitate the
analysis of antigen-specific cellular and humoral immune responses, the Integrative Multi-Omics
Core will serve as a resource to standardize, optimize, and perform multi-omic single cell research
to support the biological research objectives of Projects 1, 2 and 3. The implementation of highly
standardized and automated assays will enable longitudinal and cross-sectional analysis of the
human samples within and across the research projects. Specifically, the core will provide: i.
standardized high throughput flow cytometry, bulk transcriptomics and plasma proteomics; ii. well
established synergistic assays for the analysis of antigen-specific T cell and B cell responses,
including several orthogonal approaches for profiling antigen-specific T cells and a range of
systems serology assays; and iii. optimized cost-efficient single-cell sequencing multi-omics
methods for analysis of cellular protein-expression, gene-expression, adaptive receptor
sequences, antigen-specificity, and epigenomics based on the 10x Genomics platform.
Furthermore, we will use a collaborative cloud environment to support data ingest, processing
and provision to the DMAC for deposition into ImmPort in support of rapid public access and
innovation in data integration and visualization.

## Key facts

- **NIH application ID:** 10419583
- **Project number:** 2U19AI128914-07
- **Recipient organization:** SEATTLE CHILDREN'S HOSPITAL
- **Principal Investigator:** Peter Skene
- **Activity code:** U19 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $650,701
- **Award type:** 2
- **Project period:** 2017-07-19 → 2027-04-30

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10419583

## Citation

> US National Institutes of Health, RePORTER application 10419583, Immune Responses to Malaria, HIV and SARS-CoV-2 Infection and Immunization - Service Core (2U19AI128914-07). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10419583. Licensed CC0.

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