# Shared Resource: Cell Culture Facility

> **NIH NIH P30** · RESEARCH INST OF FOX CHASE CAN CTR · 2021 · $33,716

## Abstract

CELLULAR/MOLECULAR ANALYSES SHARED RESOURCE: CELL CULTURE FACILITY (CCF)
PROJECT SUMMARY/ABSTRACT
The Cell Culture Facility (CCF) serves as a customized pay-for-service facility with the primary goal of
providing expertise, technical support, reagents, equipment, liquid nitrogen storage, and training to increase
the effectiveness and efficiency of using mammalian cell for FCCC Cancer Center members. The CCF, with
the direction of Kerry Campbell, PhD (BCDF) and a staff of 4.5 FTEs offers technical assistance and
consultation services in tissue culture techniques, centralized liquid nitrogen banking, mycoplasma screening
services, the preparation of custom and standard culture media and supplements, performance testing of fetal
bovine serum, cell propagation, and technical support and supplies for mouse embryonic stem (ES) cell
transfection and culture. Major efforts by the CCF include carrying out technically challenging techniques, such
as the establishment of primary cell cultures from patient or animal tissue samples, hybridoma fusion and
cloning services to generate monoclonal antibodies, and culturing and manipulating ES cells to generate
genetically manipulated mice. Cell culture technology plays an essential role in modern cancer research, and is
used in a diverse array of laboratories as evidenced by the 48 Cancer Center members who used the CCF in
calendar year 2014. 95% of service hours logged for specialized cell culture services during 2014 were in
support of peer-review funded research. 68 Cancer Center members from all 5 Research Programs used the
CCF in the period 2011-2014. Importantly, the Facility supplies the FCCC community with valuable on-site
expertise for a host of established cell culture protocols to assure quality control, expert consultation in the
design and evaluation of experiments, training in cell culture methodologies, high quality culture media and
supplements at reduced costs, bulk purchase pricing of high quality performance-tested fetal bovine serum,
and the capacity to deliver personalized assistance and custom-prepared culture supplies in a timely and cost-
effective manner. Purchasing serum in bulk via the CCF saves FCCC $81,000 per year. Campbell has directed
the CCF for 15 years. Campbell's leadership prompted an institutional investment of $243,000 for new
equipment and renovation/expansion of the CCF. This renovation has significantly improved physical
infrastructure by localizing all operations in one central location, improving operational efficiencies for the
Facility staff, purchasing new and upgraded equipment, and providing a cleaner working environment to
perform tissue culture under sterile conditions. The CCF is advised by a dedicated Facility Advisory Committee
(FAC) that meets annually. Recommendations from the FAC are directed to a governing Facility Parent
Oversight Committee that serves to ensure that the CCF continues to have the necessary resources to provide
the highest quality cell cult...

## Key facts

- **NIH application ID:** 10427532
- **Project number:** 3P30CA006927-55S5
- **Recipient organization:** RESEARCH INST OF FOX CHASE CAN CTR
- **Principal Investigator:** Kerry S Campbell
- **Activity code:** P30 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $33,716
- **Award type:** 3
- **Project period:** 1997-07-01 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10427532

## Citation

> US National Institutes of Health, RePORTER application 10427532, Shared Resource: Cell Culture Facility (3P30CA006927-55S5). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10427532. Licensed CC0.

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