# Bioanalytical Core

> **NIH NIH P20** · UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA · 2022 · $350,307

## Abstract

Bioanalytical Core Abstract 
The Bioanalytical Core of CDSI will assist the COBRE Target and Pilot Project faculty and their lab personnel to 
pursue high-quality research including microRNA and epigenetic pathway analysis in immune cells following 
treatment with the dietary supplements in various models of inflammatory disease. Epigenetic studies being 
recent and cutting-edge, are more complex requiring state-of-the-art and expensive equipment, unique 
experimental parameters and data analytics experience, thereby requiring experienced operators dedicated to 
the technology. Thus, the Bioanalytical Core will serve as a technical expertise resource for the COBRE 
Target/Pilot Faculty and lab personnel. The Core will also assist the investigators with designing experiments, 
operating equipment, and trouble-shooting, aimed at helping advance research within the COBRE. The following 
specific aims will be pursued through this Core: 1) The Bioanalytical Core, will ensure purity and stability of any 
botanicals to be studied at the COBRE such as indole-3-carbinol (I3C), resveratrol (RES) and andrographolide 
(ANDR) currently being proposed in the COBRE as well as any future botanicals or plant extracts that will be 
used by the junior target faculty as well as those receiving pilot project grants. 2) To determine microRNA and 
mRNA expression profile using microarray-based approaches and perform pathway analysis to identify target 
genes following treatment with various botanicals. 3) To assist in performing genomic/epigenomic and 
microbiome research using next generation sequencing platforms, thereby helping to understand the 
mechanisms of inflammation and potential identification of biomarkers of disease. 4) To provide access to natural 
product library aimed at identifying novel anti-inflammatory compounds. 5) To provide statistical analysis for 
complex data generated using OMICS technology, which will be archived for analysis and sharing to enhance 
collaborations. In summary, the Bioanalytical Core will provide to the junior Target Faculty as well as Pilot Project 
Faculty, complete access to a wide range of state-of-the-art equipment, epigenetic assays, quality assurance for 
all botanicals tested at the CDSI, access to new natural products with potential anti-inflammatory properties, 
insights into the epigenetic and molecular basis of induction of disease, and help identify potential biomarkers 
and molecular signatures of pathogenesis. Together, this Core will be highly innovative value-added shared 
resource critical for advancing and sustaining the goals of the CDSI.

## Key facts

- **NIH application ID:** 10428527
- **Project number:** 5P20GM103641-10
- **Recipient organization:** UNIVERSITY OF SOUTH CAROLINA AT COLUMBIA
- **Principal Investigator:** NARENDRA P SINGH
- **Activity code:** P20 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $350,307
- **Award type:** 5
- **Project period:** 2012-09-01 → 2025-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10428527

## Citation

> US National Institutes of Health, RePORTER application 10428527, Bioanalytical Core (5P20GM103641-10). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10428527. Licensed CC0.

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