# Examining how the novel S64F MAFA variant produces glucose intolerance or hypoglycemia in a sex-dependent manner

> **NIH NIH K08** · VANDERBILT UNIVERSITY MEDICAL CENTER · 2022 · $158,316

## Abstract

PROJECT ABSTRACT
As of 2018, 30.3 million Americans have been diagnosed with diabetes (10% of the U.S. population with a
male sex bias). Its close associations with many chronic diseases, such as heart attacks, strokes, and cancers,
make diabetes a leading risk factor for morbidity and mortality. In all forms of diabetes, the inability to maintain
normal glucose levels results from progressive dysfunction and eventual loss of insulin-producing b-cells in the
pancreas. With high rates of treatment failure on standard therapy, developing new therapeutic approaches to
preserve or even enhance b-cell function is a priority. Furthermore, differences in metabolism between men
and women during healthy aging and disease are appreciated but poorly understood.
Pancreatic b-cells require several key factors to appropriately secrete insulin. One such factor is MafA, a
transcription factor fundamental to mature b-cell function. The early loss of human MafA (MAFA) in b-cells in
patients with type 2 diabetes highlights its importance to human b-cell health. In addition, a naturally occurring,
genetic mutation in MAFA (S64F MAFA) was recently identified to predispose carriers to either familial, adult-
onset diabetes or hypoglycemia (low blood glucose). Curiously, S64F MAFA-associated diabetes is much
more prevalent in men while women tend to present with hypoglycemia. To better understand the sex-
dependent effects of this variant, we generated a mouse model harboring this mutation. This model shows the
expected sex-dependent effects seen in humans, suggesting similar mechanisms between mice and humans.
Male S64F MAFA mice were hyperglycemic due to widespread, premature b-cell aging and senescence, while
female S64F MAFA mice were hypoglycemic by a mechanism which is not yet clearly defined. However, our
preliminary studies suggest that S64F MAFA creates different b-cell subtypes in females, one of which is
hyperfunctional. Taken together, these results suggest that S64F MAFA can incur diverse b-cell responses to
produce sex-dependent diseases: diabetes (b-cell hypofunction) and hypoglycemia (b-cell hyperfunction).
This investigation will identify and compare the diverse molecular responses to S64F MAFA in male and
female b-cells across mice and humans to understand the sex-dependent, b-cell responses unique to human
b-cells. We will first use the penetrant, proof-of-principle S64F MAFA mouse model which mimics several
aspects of human disease to identify the diverse b-cell populations by single cell transcriptomics. For example,
diversity in premature aging signatures will be related to the dysfunction seen in senescent, male S64F MAFA
b-cells. We will then investigate the molecular and functional responses to the S64F MAFA protein in
genetically modified, male and female human b-cells using novel pseudoislet technology to identify targets
unique to human b-cell function. In sum, our work will advance fundamental understanding of sex-dependent
b-cell responses...

## Key facts

- **NIH application ID:** 10428958
- **Project number:** 1K08DK132507-01
- **Recipient organization:** VANDERBILT UNIVERSITY MEDICAL CENTER
- **Principal Investigator:** Jeeyeon Cha
- **Activity code:** K08 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2022
- **Award amount:** $158,316
- **Award type:** 1
- **Project period:** 2022-07-01 → 2027-05-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10428958

## Citation

> US National Institutes of Health, RePORTER application 10428958, Examining how the novel S64F MAFA variant produces glucose intolerance or hypoglycemia in a sex-dependent manner (1K08DK132507-01). Retrieved via AI Analytics 2026-05-22 from https://api.ai-analytics.org/grant/nih/10428958. Licensed CC0.

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