# De novo production of new knockout mouse lines for the next phase of the KOMP2 Project

> **NIH NIH UM1** · UNIVERSITY OF CALIFORNIA AT DAVIS · 2021 · $159,953

## Abstract

De novo production of new knockout mouse lines for the next phase of the KOMP2 Project
UM1OD023221 Administrative Supplement
August 1, 2021 – July 31, 2022
ABSTRACT
The aim of this administrative supplement request is to generate 25 new homozygous viable
gene knockout mouse lines ready for production of male and female cohorts for phenotyping in
a forthcoming KOMP2-Phase3 project. To do so, this supplement will fund production activities
under 3 specific aims. Specific Aim 1 involves gene selection and exdel KO allele design,
CRISPR guide in vitro quality control (QC), zygote electroporation, embryo transfer, gestation
and parturition, founder (F0) screening by gene specific PCR genotyping, weaning and housing
F0 mice until breeding age, F0 breeding and sequence quality control (QC) to confirm germline
transmission (GLT) for 25 genes (18 at UC Davis, 7 at TCP). Specific Aim 2 involves QC of N1
mice, production reporting to GenTar, and line expansion of N2 mice to breeding age which will
be ready to generate male and female cohorts for phenotyping. Specific Aim 3 involves sperm
cryopreservation of 2 heterozygote males for each line produced and deposition into the
MMRRC at UC Davis. This work will be completed by July 31, 2022.

## Key facts

- **NIH application ID:** 10429218
- **Project number:** 3UM1OD023221-10S2
- **Recipient organization:** UNIVERSITY OF CALIFORNIA AT DAVIS
- **Principal Investigator:** KC KENT LLOYD
- **Activity code:** UM1 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $159,953
- **Award type:** 3
- **Project period:** 2011-09-16 → 2022-07-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10429218

## Citation

> US National Institutes of Health, RePORTER application 10429218, De novo production of new knockout mouse lines for the next phase of the KOMP2 Project (3UM1OD023221-10S2). Retrieved via AI Analytics 2026-05-24 from https://api.ai-analytics.org/grant/nih/10429218. Licensed CC0.

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