# A Community Knowledgebase to Evaluate Host-Microbial Salivary Proteomics

> **NIH NIH R01** · ADA FORSYTH INSTITUTE, INC. · 2021 · $488,025

## Abstract

ABSTRACT
An important goal of the oral, dental and craniofacial biology research community is to understand how host and
microbes interactions work within each niche and disseminate the data widely. Because of saliva’s complexity,
biofluid-based proteomic evaluations provide new resources and knowledge related to the composition and
function. The Human Salivary Proteome Wiki (HSP Wiki) was developed with the aim to allow the oral research
community to expand the available information. Yet, to achieve real advance and capture the molecular
complexity of saliva, novel resources must be put into place. The investigations outlined on this proposal are
poised to ultimately provide novel information on host-microbial interactions in the oral cavity by harnessing
information gained from the salivary proteome. The Long-Term Goal of this project is to establish the resources
and develop new tools to allow continuity in salivary research for both scientific discovery and diagnostic
applications. For that purpose, the First Aim will transfer and provide the research community with a functional
and curated database that is reliable and innovative to evaluate proteomic studies. As a community resource for
depositing, annotating, and studying salivary proteomic data, the HSP Wiki platform has a goal to provide
access to of an array of information and analytic tools. The Second Aim will implement a novel computational
infrastructure for salivary proteomic datasets that includes visual analytics, faster processing, and efficient
measures of success. The Third Aim will utilize the Human Oral Microbiome Database (HOMD) to provide web-
accessible taxonomic, genomic and proteomic information and analysis tools for the research community.
Because it is currently being expanded to include information on the viruses (phages) that infect the oral bacteria
and archaea, we aim to integrate proteome data from HOMD into the HSP Wiki database. Here, we aim to
perform a series of studies to determine how the salivary proteome identification coverage is increased by
expanding the proteome reference library from just human proteins to those derived from oral bacteria, archaea
and phages. The expected outcome of HSP and HOMD integration will provide the research community with a
greatly enhanced online resource for examining the composition of the salivary proteome and its impact on
human health and disease. Broad dissemination of this project and increased use of the HSP Wiki will catalyze
discovery, enhance rigor, and will eventually increase chances of translation towards precision medicine and
diagnostic applications of saliva-derived biomarkers.

## Key facts

- **NIH application ID:** 10429378
- **Project number:** 3R01DE016937-16S1
- **Recipient organization:** ADA FORSYTH INSTITUTE, INC.
- **Principal Investigator:** Floyd E Dewhirst
- **Activity code:** R01 (R01, R21, SBIR, etc.)
- **Funding institute:** NIH
- **Fiscal year:** 2021
- **Award amount:** $488,025
- **Award type:** 3
- **Project period:** 2021-07-01 → 2026-03-31

## Primary source

NIH RePORTER: https://reporter.nih.gov/project-details/10429378

## Citation

> US National Institutes of Health, RePORTER application 10429378, A Community Knowledgebase to Evaluate Host-Microbial Salivary Proteomics (3R01DE016937-16S1). Retrieved via AI Analytics 2026-05-23 from https://api.ai-analytics.org/grant/nih/10429378. Licensed CC0.

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